Exploring the neuroprotective effects of black garlic ethanol extract on acrylamide-induced brain damage through apoptotic and neurodegenerative pathways

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dc.authoridozkaraca, mustafa/0000-0002-6359-6249
dc.authoridCalik, Fatma/0000-0003-1548-8689
dc.authoridBedir, Gursel/0000-0001-8859-7814
dc.authoridgezer, arzu/0000-0002-1658-2098
dc.authoridelis, ISA/0000-0003-3212-7842
dc.contributor.authorGezer, Arzu
dc.contributor.authorKaradag Sari, Ebru
dc.contributor.authorGelen, Volkan
dc.contributor.authorElis Yildiz, Sevda
dc.contributor.authorOzkaraca, Mustafa
dc.contributor.authorBedir, Gursel
dc.contributor.authorCalik, Fatma
dc.date.accessioned2025-05-04T16:45:39Z
dc.date.available2025-05-04T16:45:39Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis research focused on exploring the therapeutic impact of black garlic ethanol extract (BGE) on the brain tissue of rats exposed to acrylamide (ACR). Twenty-four female rats were divided into four groups. Rats in the control group were given 1 ml of saline by oral gavage for 14 days. The BG group received 5 mg/200 g of BGE extract on a daily basis. The ACR group was administered 40 mg/kg of ACR daily. Rats in the BGE+ACR group received both 5 mg/200 g of BG extract and 40 mg/kg of ACR daily. Brain tissue samples were collected at the study's conclusion for histopathological, immunohistochemical, and biochemical analyses. Hematoxylin-eosin staining was performed to examine the general structure of the brain tissue. Erk1/2, pERK1/2, and c-fos were analyzed immunohistochemically; Bcl-2, Caspase-3, ATF6, CREB, and NfkB-p65 protein levels were analyzed by Western blotting; and MDA, SOD, CAT, GSH, TNF-alpha, IL-1(3, and IL-6 activities and levels were analyzed using ELISA kits. It was determined that ACR application raised the levels of Erk1/2, p-ERK1/2, c-Fos, NfkB-p65, caspase-3, MDA, IL-6, IL-1-(3, and TNF-alpha, and BGE supplementation decreased this increase. ACR exposure caused a decrease in Bcl-2, ATF6, CREB, CAT, GSH, and SOD expressions, and BGE supplementation prevented or increased this decrease. Based on the findings obtained, it can be said that the ethanol extract of black garlic has antioxidative and anti-inflammatory effects, prevents cell damage, and has positive effects on apoptosis in rat brain tissue.
dc.identifier.doi10.33988/auvfd.1384531
dc.identifier.endpage406
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85210817643
dc.identifier.scopusqualityQ3
dc.identifier.startpage395
dc.identifier.urihttps://doi.org/10.33988/auvfd.1384531
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35172
dc.identifier.volume71
dc.identifier.wosWOS:001338109700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAnkara Univ
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250504
dc.subjectAcrylamide
dc.subjectApoptosis
dc.subjectBlack Garlic
dc.subjectNeurodegeneration
dc.subjectNeurotoxicity
dc.titleExploring the neuroprotective effects of black garlic ethanol extract on acrylamide-induced brain damage through apoptotic and neurodegenerative pathways
dc.typeArticle

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