Investigation of the effect of REM sleep deprivation on epileptic seizures caused by pentylenetetrazole in mice

Aim: To investigate whether different periods of rapid eye movement sleep deprivation (REM SD)contribute to seizure susceptibility, hippocampal oxidative status and balance of inhibition-excitationin the acute epilepsy model.Methods: REM SD was performed using the modified multiple platforms method on adult maleBALB/c mice. Pentylentetrazol (PTZ) was injected to induce seizures and hippocampal totalantioxidant status (TAS), total oxidant status (TOS), gamma aminobutyric acid (GABA), andglutamate levels were measured using the ELISA method.Results: PTZ-induced seizures following 8 h and 72 h REM SD significantly reduced thehippocampal TAS levels, but did not affect the TOS levels. In REM SD groups, especially after 8hours of REM sleep loss, there was a significant increase in glutamate in PTZ induction. Thehippocampal GABA levels were increased by PTZ-induced seizures after 72 h REM SD. PTZinduction after 8 hours of RAM SD leads to a significant increase in the seizure duration.Conclusion: It can be speculated that the REM SD can contribute to seizure susceptibility bychanging the oxidant-antioxidant balance and excitatory and inhibitory tone in the hippocampus.