Investigation of Cytotoxic and Antimicrobial Effects of Polyanhydride-Based Poly[(maleic anhydride)-co-(vinyl Acetate)] Conjugates Combined with Methotrexate and Gemcitabine in Breast Cancer Treatment

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dc.authoridTUNC, Tutku/0000-0002-8274-9386
dc.contributor.authorTunc, Tutku
dc.contributor.authorKarakus, Gulderen
dc.contributor.authorSumer, Zeynep
dc.date.accessioned2025-05-04T16:46:57Z
dc.date.available2025-05-04T16:46:57Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractStudies aimed at increasing the efficacy of chemotherapeutic drugs and reducing or completely eliminating their side effects are frequently encountered. In our study, we considered methotrexate (MTX), which is in the category of anticarcinogenic and anti-inflammatory drugs, and gemcitabine (GEM), which is used in the treatment of breast, testicular, ovarian, etc. cancers. GEM, which is used in the treatment of breast, testicular, ovarian, etc. cancers, was covalently bonded to maleic anhydride vinyl acetate (MAVA) copolymer, and new polymer-drug conjugates (MAVA-MTX and MAVA-GEM) were obtained to reduce or eliminate the side effects of these drugs and to investigate the cytotoxic and antimicrobial effects of the new conjugates. The conjugation reaction was carried out in the presence of a triethylamine catalyst in dimethylformamide medium at 70 degrees C. Chemical structure elucidation of the copolymer (MAVA) and conjugates (MAVA-MTX and MAVA-GEM) was carried out by Fourier transform infrared (FTIR) and Nuclear Magnetic Resonance (1H NMR) spectroscopy. Anticancer activity was determined by the MTT assay in MCF-7 (breast cancer), and L929 (mouse fibroblast) cell lines. The synthesized copolymer and conjugate structures were proved by FTIR and 1H NMR spectra. It was determined that the conjugates did not form an inhibition zone on the test microorganisms. MIC values were found to be moderately effective compared with reference sources. The anticancer activities of MAVA-MTX and MAVA-GEM conjugates were significantly higher than those of methotrexate and GEM. The higher anticancer activity of the synthesized MAVA-MTX and MAVA-GEM conjugates compared with the drug they contain suggests that they can be a potential drug candidate in the treatment of breast cancer. In addition, the conjugates showed less toxic effect on a healthy L929 cell line at 6 different concentrations compared to free drugs. This can be shown as a significant improvement in reducing one of the most important side effects of the drug, such as toxicity.
dc.description.sponsorshipSivas Cumhuriyet ?niversitesi [T-458]; CUBAP
dc.description.sponsorshipWe gratefully acknowledge the support of CUBAP (T-458) for the anticancer and antimicrobial activity assay of this research.
dc.identifier.doi10.1021/acsomega.4c10445
dc.identifier.endpage12162
dc.identifier.issn2470-1343
dc.identifier.issue12
dc.identifier.pmid40191318
dc.identifier.scopus2-s2.0-105003031032
dc.identifier.scopusqualityQ1
dc.identifier.startpage12152
dc.identifier.urihttps://doi.org/10.1021/acsomega.4c10445
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35431
dc.identifier.volume10
dc.identifier.wosWOS:001450167100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Omega
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250504
dc.subjectDrug
dc.subjectHydrochloride
dc.titleInvestigation of Cytotoxic and Antimicrobial Effects of Polyanhydride-Based Poly[(maleic anhydride)-co-(vinyl Acetate)] Conjugates Combined with Methotrexate and Gemcitabine in Breast Cancer Treatment
dc.typeArticle

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