IMMUNOFLUORESCENCE AND BIOCHEMICAL INVESTIGATION OF THE PROTECTIVE EFFECTS OF NARINGIN AND DIOSMIN ON THE FREEZABILITY OF MERINO RAM SEMEN

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dc.contributor.authorOmuer, Ali Dogan
dc.contributor.authorKaynar, Ozgur
dc.contributor.authorIleriturk, Mustafa
dc.contributor.authorOzkaraca, Mustafa
dc.contributor.authorOzturk, Ali Erdem
dc.contributor.authorBodu, Mustafa
dc.contributor.authorSahin, Ipek Nur
dc.date.accessioned2024-10-26T18:10:50Z
dc.date.available2024-10-26T18:10:50Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBACKGROUND: Various antioxidant substances are added to sperm extenders to protect spermatozoa against oxidative stress and cryodamage. OBJECTIVE: To investigate the effects of the flavonoid diosmin (DIO) and a flavanone glycoside naringin (NAR) on the freezability of ram semen. MATERIALS AND METHODS: In this study, six Merino rams were used during the breeding season. The ejaculates were pooled after collection from the rams. Pooled ejaculates were divided into six groups: control, NAR 1 mM, NAR 2 mM, NAR 4 mM, DIO 2 mM, and DIO 4 mM, and then diluted with a TRIS-based diluent. The pooled semen was equilibrated, placed in 0.25 mL pipettes with 10 x 10(7) sperm cells in each pipette, and frozen in liquid nitrogen vapor. After 24 h, the pipettes were thawed at 37 degrees C for 25 s and analyzed in terms of spermatological parameters. RESULTS: The highest plasma membrane integrity ratio was found in the DIO 4 mM group, whereas a statistically significant difference was found between the NAR 1 mM and NAR 2 mM groups (p<0.05). While the DIO 4 mM group had the highest acrosome integrity rate, a statistically significant difference was found between the other groups (p<0.05). Mitochondrial activity was the highest in the NAR 4 mM, DIO 4 mM and DIO 2 mM groups (p<0.05). In the analysis of the sperm membrane lipid profile, it was observed that the DIO group had the highest lipid-phospholipid ratio. In sperm membrane protein profile analysis, it was found that both additives exerted protective effects at different levels. The highest total protein content was seen in the DIO 4 mM and NAR 4 mM groups. 8hydroxydeoxyguanosine (8-OhDG) positivity was more common in the control group than in the DIO and NAR groups. Cu-Zn superoxide dismutase (SOD) expression was lower in the control group and more intense in all other groups. Positive results were especially observed in the acrosome of the sperm cells. CONCLUSION: The addition of NAR and DIO to the ram semen extender increased the quality of sperm parameters after the freeze-thaw process.
dc.description.sponsorshipTUBITAK [116O604 -3001]
dc.description.sponsorshipThe study work entitled Immunofluorescence and Biochemical Investigation of the Protective Effects of naringin and Diosmine on the Freezability of Merino Ram Semen was financially supported by TUBITAK (116O604 -3001).
dc.identifier.doi10.54680/fr24510110412
dc.identifier.endpage319
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85201064330
dc.identifier.scopusqualityQ3
dc.identifier.startpage309
dc.identifier.urihttps://doi.org/10.54680/fr24510110412
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30400
dc.identifier.volume45
dc.identifier.wosWOS:001304170800006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcryopreservation
dc.subjectdiosmin
dc.subjectlipid / protein profile analysis
dc.subjectnaringin
dc.subjectsperm
dc.titleIMMUNOFLUORESCENCE AND BIOCHEMICAL INVESTIGATION OF THE PROTECTIVE EFFECTS OF NARINGIN AND DIOSMIN ON THE FREEZABILITY OF MERINO RAM SEMEN
dc.typeArticle

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