Perturbation of HSP Network in MCF-7 Breast Cancer Cell Line Triggers Inducible HSP70 Expression and Leads to Tumor Suppression

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dc.authoridYILDIZ, Mehmet Taha/0000-0003-4768-0333
dc.authoridTUTAR, Yusuf/0000-0003-2613-9644
dc.authoridSERT, YUSUF/0000-0001-8836-8667
dc.authoridErgul, Mustafa/0000-0003-4303-2996
dc.contributor.authorErgul, Mustafa
dc.contributor.authorAktan, Fugen
dc.contributor.authorYildiz, Mehmet T.
dc.contributor.authorTutar, Yusuf
dc.date.accessioned2024-10-26T18:09:02Z
dc.date.available2024-10-26T18:09:02Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground: Heat shock protein 70 (HSP70) is constitutively expressed in normal cells but aberrantly expressed in several types of tumor cells, helping their survival in extreme conditions. Thus, specific inhibition of HSP70 in tumor cells is a promising strategy in the treatment of cancer. HSP70 has a variety, of isoforms in the cellular organelles and form different functions by co-ordinating and cooperating with co chaperones. Cancer cells overexpress HSPs during cell growth and proliferation and HSP network provides resistance against apoptosis. The present study aimed to evaluate quantitative changes in HSPs- and cancer-associated gene expressions and their interactions in the presence of 2-phenylethyenesulfonamide (PES) in MCF-7 cells. Methods: AntiproliCerative activity of PES was evaluated using the wXTT assay. Inducible HSP70 (HSP70i) levels in the PES-treated cells were determined using the ELISA kit. PCR Array was performed to assess the IISPs- and cancer-pathway focused gene expression profiling. Gene network analysis was performed using the X2K, yLd (N1.3.18.1) programs, and web-based gene list enrichment analysis tool Enrichr. Results: The results demonstrated that PES exposure increased the amount of both HSP70i gene and protein expression surprisingly. However, the expression of HSP70 isoforms as well as other co-chaperones, and 17 cancer-associated genes decreased remarkably as expected. Additionally, interaction network analysis revealed a different mechanism; PES induction of HSP70i employs a cell cycle negative regulator, RBI, which is a tumor suppressor gene. Conclusion: PES treatment inhibited MCF-7 cell proliferation and changed several HSPs- and cancer-related gene expressions along with their interactions through a unique mechanism although it causes an interesting increase at HSP70i gene and protein expressions. RBI gene expression may play an important role in this effect as revealed by the interaction network analysis.
dc.description.sponsorshipTubitak [114Z365]; Ankara University [15L0237006]
dc.description.sponsorshipThis work is supported by Tubitak (grant #114Z365) and a mini - grant from Ankara University (grant #15L0237006).
dc.identifier.doi10.2174/1871520620666200213102210
dc.identifier.endpage1060
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue9
dc.identifier.pmid32053081
dc.identifier.scopus2-s2.0-85088935039
dc.identifier.scopusqualityQ3
dc.identifier.startpage1051
dc.identifier.urihttps://doi.org/10.2174/1871520620666200213102210
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29917
dc.identifier.volume20
dc.identifier.wosWOS:000563773700002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofAnti-Cancer Agents in Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHSP70
dc.subjectpifithrin-mu
dc.subjectPCR array
dc.subjecthuman breast cancer cell
dc.subjectELISA
dc.subjectPES induction
dc.titlePerturbation of HSP Network in MCF-7 Breast Cancer Cell Line Triggers Inducible HSP70 Expression and Leads to Tumor Suppression
dc.typeArticle

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