Isolation and molecular identification of cellulose-degrading bacteria from rumen sheep Ovis aries and evaluation of their cellulase production

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dc.contributor.authorBen Ghalib, Khaoula
dc.contributor.authorChadli, Mohamed
dc.contributor.authorDastan, Sevgi Durna
dc.contributor.authorElmtili, Noureddine
dc.date.accessioned2025-05-04T16:47:00Z
dc.date.available2025-05-04T16:47:00Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study focuses on the isolation and identification of cellulose-degrading bacteria from the digestive tract of sheep ( Ovis aries) and to determine the cellulase capacity of different isolates for ulterior application. The bacterial strains were screened firstly using Congo red, where cellulase activity is indicated by the appearance of a hydrolysis zone on the Carboxymethylcellulose medium (CMC), the clearance zone value ranged between 8 and 15 mm for all isolates and the hydrolytic capacity was between 1.6 to 2.5 mm. The cellulolytic strain isolates were identified using MALDI-TOF Mass Spectrometry and 16S ribosomal RNA gene sequencing. MALDI-TOF MS test indicated that all strains belonged to Bacillaceae family. The strains Isolate2, Isolate3, and Isolate8 were reliable to B. tequilensis species with a score value 2.0. Whereas, the Isolate1, Isolate4, Isolate5, and Isolate6 were reliably identified to the genus level (Score value 1.7-1.99). The molecular identification results revealed that the strains indicate a high sequence similarity with 16S rRNA gene sequences accessible in GenBank database and belong to Priestia megaterium, Lysinibacillus capsici, Bacillus tequilensis, and Bacillus paralicheniformis. The highest Carboxymethylcellulase activity (CMCase) was obtained by B. tequilensis (0.827 +/- 0.035 U/ml) at a pH of 7.0 and temperature of 30 degrees C at 100 g. The carbon source utilized was CMC (1 %), while peptone (1 %) and ammonium sulfate (0.24 %) served as the nitrogen sources. Further research in optimizing and purifying of cellulase, could be useful for the future hydrolyzation of some green biomass for various biotechnological applications such as biofuel production.
dc.description.sponsorshipScientific Research Committee of Sivas Cumhuriyet University [F-619]
dc.description.sponsorshipScientific Research Committee of Sivas Cumhuriyet University supported a small part of this study within the framework of bilateral agreement under the Project number of F-619.
dc.identifier.doi10.1016/j.sciaf.2024.e02439
dc.identifier.issn2468-2276
dc.identifier.scopus2-s2.0-85206798948
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.sciaf.2024.e02439
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35451
dc.identifier.volume26
dc.identifier.wosWOS:001340660800001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofScientific African
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250504
dc.subjectBacillaceae
dc.subject16S-rRNA
dc.subjectMALDI-TOF MS
dc.subjectCellulase
dc.subjectCellulolytic bacteria
dc.subjectCMCase
dc.titleIsolation and molecular identification of cellulose-degrading bacteria from rumen sheep Ovis aries and evaluation of their cellulase production
dc.typeArticle

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