Evaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior

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dc.authoridBudak, Harun/0000-0002-7371-8959
dc.authoridozkaraca, mustafa/0000-0002-6359-6249
dc.contributor.authorToraman, Ayse
dc.contributor.authorToraman, Emine
dc.contributor.authorOzkaraca, Mustafa
dc.contributor.authorBudak, Harun
dc.date.accessioned2024-10-26T18:06:02Z
dc.date.available2024-10-26T18:06:02Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground Oxidative stress has a critical effect on both persistent pain states and periodontal disease. Voltage-gated sodium NaV1.7 (SCN9A), and transient receptor potential ankyrin 1 (TRPA1) are pain genes. The goal of this study was to investigate oxidative stress markers, periodontal status, SCN9A, and TRPA1 channel expression in periodontal tissues of rats with paclitaxel-induced neuropathic pain-like behavior (NPLB). Methods and results Totally 16 male Sprague Dawley rats were used: control (n=8) and paclitaxel-induced pain (PTX) (n=8). The alveolar bone loss and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were analyzed histometrically and immunohistochemically. Gingival superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities (spectrophotometric assay) were measured. The relative TRPA1 and SCN9A genes expression levels were evaluated using quantitative real-time PCR (qPCR) in the tissues of gingiva and brain. The PTX group had significantly higher alveolar bone loss and 8-OHdG compared to the control. The PTX group had significantly lower gingival SOD, GPx and CAT activity than the control groups. The PTX group had significantly higher relative gene expression of SCN9A (p=0.0002) and TRPA1 (p=0.0002) than the control in gingival tissues. Increased nociceptive susceptibility may affect the increase in oxidative stress and periodontal destruction. Conclusions Chronic pain conditions may increase TRPA1 and SCN9A gene expression in the periodontium. The data of the current study may help develop novel approaches both to maintain periodontal health and alleviate pain in patients suffering from orofacial pain.
dc.identifier.doi10.1007/s11033-023-08829-z
dc.identifier.endpage9322
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue11
dc.identifier.pmid37812355
dc.identifier.scopus2-s2.0-85173962956
dc.identifier.scopusqualityQ2
dc.identifier.startpage9315
dc.identifier.urihttps://doi.org/10.1007/s11033-023-08829-z
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29326
dc.identifier.volume50
dc.identifier.wosWOS:001083288000007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectChronic pain
dc.subjectOxidative stress
dc.subjectNociceptive sensitivity
dc.subjectPeriodontal disease
dc.subjectSCN9A
dc.subjectTRPA1
dc.titleEvaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior
dc.typeArticle

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