Development of a novel triplex-PCR assay for the identification of feline hemoplasma species and survey of hemoplasma species in cats in Turkiye

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dc.contributor.authorAltay, Kursat
dc.contributor.authorCoskun, Alparslan
dc.contributor.authorErol, Ufuk
dc.contributor.authorSahin, Omer Faruk
dc.contributor.authorTurk, Sefer
dc.date.accessioned2024-10-26T18:02:44Z
dc.date.available2024-10-26T18:02:44Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThree hemoplasma species, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum, and Candidatus Mycoplasma turicensis, have been identified in domestic and wild felids. M. haemofelis may cause severe clinical manifestations in domestic cats, whereas others can lead to mild infections. Identification of these pathogens is done using molecular diagnostic tools like conventional-PCR or real-time PCR. However, these have disadvantages, such as the failure to differentiate species or high cost. This study aimed to develop a triplex-PCR method for the diagnosis and discrimination of feline hemoplasma species. Furthermore, it is aimed at providing molecular data on the epidemiology of feline hemoplasma species in Turkiye, where there is a paucity of information on these pathogens. Triplex-PCR primers amplifying the 16S rRNA gene regions of M. haemofelis (1022 bp), Ca. Mycoplasma haemominutum (607 bp), and Ca. Mycoplasma turicensis (456 bp) species were designed and optimized. Moreover, the detection limits of the method were also determined and it was found that the primers could detect 0.001 ng/mu L amount of DNA for M. haemofelis, 0.0001 ng/mu L for Ca. Mycoplasma haemominutum, and 0.0002 ng/mu L for Ca. Mycoplasma turicensis in the sample. 286 cat blood samples obtained from Sivas province were researched for feline hemoplasma species. Feline hemoplasma species were detected in samples of 29 out of 286 cats (10.23 %). Five samples (1.74 %) were infected with only M. haemofelis, whereas 22 (7.69 %) were only infected with Ca. Mycoplasma haemominutum. Two samples (0.69 %) were found to be infected with both M. haemofelis and Ca. Mycoplasma haemominutum. Ca. Mycoplasma turicensis was not detected in this study. A triplex-PCR method that can be used for the identification and species differentiation of feline hemoplasma species in hosts was developed. Moreover, hemoplasma species were found to be circulating in cats in the study area, and it is recommended that veterinarians and animal owners take the necessary precautions to protect themselves and their cats from these pathogens.
dc.description.sponsorshipThe authors would like to thank all veterinarians and technicians for their kind help during sample collection. Part of this study was presented as an oral presentation at the 23rd National Parasitology Congress in Turkiye.
dc.identifier.doi10.1016/j.parint.2024.102969
dc.identifier.issn1383-5769
dc.identifier.issn1873-0329
dc.identifier.pmid39276922
dc.identifier.scopus2-s2.0-85204183110
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.parint.2024.102969
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28328
dc.identifier.volume104
dc.identifier.wosWOS:001320052100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofParasitology International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHemotropic Mycoplasma
dc.subjectCat
dc.subjectTriplex-PCR
dc.subject16SrRNA
dc.subjectTurkiye
dc.titleDevelopment of a novel triplex-PCR assay for the identification of feline hemoplasma species and survey of hemoplasma species in cats in Turkiye
dc.typeArticle

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