Occurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye

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dc.authoridALTAY, KURSAT/0000-0002-5288-1239
dc.authoridSAKAR, HUSNU FURKAN/0009-0009-4647-9321
dc.authoridUrhan, Osman Furkan/0000-0002-2834-3051
dc.authoridSAHIN, Omer Faruk/0000-0002-3230-504X
dc.authoridErol, Ufuk/0000-0002-6766-1335
dc.contributor.authorSahin, Omer Faruk
dc.contributor.authorErol, Ufuk
dc.contributor.authorUrhan, Osman Furkan
dc.contributor.authorSakar, Husnu Furkan
dc.contributor.authorAltay, Kursat
dc.date.accessioned2025-05-04T16:47:24Z
dc.date.available2025-05-04T16:47:24Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractPurposeCryptosporidium spp. and Giardia duodenalis are zoonotic protozoan parasites that are widely seen in domestic and wild animals worldwide. While these pathogens, which affect the digestive system of the hosts, cause high economic losses in animal breeding, they are also considered an important public health problem. In recent years, molecular-based studies revealed that 120 genotypes belonging to 44 Cryptosporidium species and eight G. duodenalis assemblages (G. duodenalis A-H) circulate among hosts. The aim of the study was to determine the presence and prevalence of cryptosporidiosis and giardiosis in buffaloes, for which there was only one previous study on the subject in T & uuml;rkiye.MethodsIn this study, Cryptosporidium spp. and Giardia duodenalis were researched in water buffaloes using polymerase chain reaction (PCR) and DNA sequencing. A total of 510 water buffalo stool samples were obtained from Sivas province, an important water buffalo breeding center in T & uuml;rkiye.ResultsCryptosporidium spp. were detected in 20 samples (3.92%), whereas five samples (0.98%) were found to be infected with G. duodenalis. DNA sequence analyses of 18S rRNA and beta-giardin genes revealed that five Cryptosporidium species, C. occultus (n = 1), C. andersoni (n = 1), C. ryanae (n = 16), C. parvum (n = 1), and C. bovis (n = 1), and G. duodenalis assemblages E were circulated in water buffaloes in T & uuml;rkiye, respectively. In this work, C. ryanae was the most prevalent Cryptosporidium species, and DNA sequence analyses of these samples showed that 100% nucleotide identities were present between them. Cryptosporidium occultus (PP754270), C. andersoni (PP754271), C. ryanae (PP754272-PP754279, PP754281-PP754285, PP754287-PP754289), C. parvum (PP754280), and C. bovis (PP754286) obtained from water buffaloes in this study shared 98.59-100%, 99.88-100%, 99.49-100%, 99.62-100%, and 99.87-100% nucleotide similarity with isolates present in GeneBank, respectively. In addition, G. duodenalis (PP798352-PP798356) isolates had 99.56-100% (beta-giardin) nucleotide identities with G. duodenalis isolates.ConclusionThe existence of cryptosporidiosis (the five species) in water buffaloes was reported for the first time in the country. Moreover, one species (C. occultus) has been reported for the first time in T & uuml;rkiye.
dc.description.sponsorshipScientific Research Project Fund of Sivas Cumhuriyet University
dc.description.sponsorshipThe authors would like to thank all veterinarians and technicians for their kind help during sample collection
dc.identifier.doi10.1007/s11686-024-00975-z
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.issue1
dc.identifier.pmid39762520
dc.identifier.scopus2-s2.0-85214379338
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1007/s11686-024-00975-z
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35596
dc.identifier.volume70
dc.identifier.wosWOS:001390548500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofActa Parasitologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectCryptosporidium
dc.subjectGiardia duodenalis
dc.subjectWater Buffalo
dc.subjectPCR
dc.subjectDNA sequencing
dc.subjectT & uuml;rkiye
dc.titleOccurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye
dc.typeArticle

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