In Silico and In Vitro Studies of Novel Azomethines on DNA Repair Genes in Gastric Cell Lines

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dc.authorid0000-0003-3433-8870tr
dc.contributor.authorOzturk, Alpaslan
dc.contributor.authorAgbektas, Tugba
dc.contributor.authorHuseynzada, Alakbar
dc.contributor.authorGuliyev, Ruslan
dc.contributor.authorGanbarova, Rana
dc.contributor.authorHasanova, Ulviyya
dc.contributor.authorTas, Ayca
dc.contributor.authorErkan Sultan
dc.contributor.authorZontul, Cemile
dc.contributor.authorInandiklioglu, Nihal
dc.contributor.authorSilig, Yavuz
dc.date.accessioned2024-03-07T07:01:27Z
dc.date.available2024-03-07T07:01:27Z
dc.date.issued2023/9/28tr
dc.departmentYıldızeli Meslek Yüksekokulutr
dc.description.abstractWe herein report the determination of the cytotoxic activity and expression profiles of some DNArepair genes of newly synthesized azomethines in the gastric cancer cell line (AGS). The studied novel compounds were synthesized by a condensation reaction and received compounds werecharacterized by 1Hand13CNMRspectroscopymethods. Furthermore, theywereappliedtothe AGScell line at eight different concentrations (0.1–50 g/mL). Anticancer activities were determined using the MTTmethod. Expressionlevels of ATR,ERCC1, TOP2A,andABCB1genesweredetermined by the RT-PCRmethod. Biochemical parameters were also examined. The interaction of proteins with other proteins was investigated with the String v11 program. The IC50 values of compounds 1, 2, and 3 obtained after 72 h were 23.10, 8.93, and 1.58 g/mL, respectively. The results demonstrate that the cytotoxic activity of compound 3 on AGS cancer cells is higher in comparison with other molecules. It was determined that the expression levels of ATR, TOP2A, and ABCB1 genes in compounds 1, 2, and 3 were decreased compared to the control group. In addition, it was determined that ERCC1 gene expression increased in compound 3, decreased in compound 2, and remained unchanged in compound 1 (p <0.001). In AGS gastric cancer cells, a 64% decrease was detected for GST levels in compound 1, while a 38% decrease in GSH levels in compound 2. In addition, compounds 1–3 were examined at the molecular level with computational techniques and the docking studies revealed 4LN0 as a target proteintr
dc.identifier.doi10.3390/life13101982en_US
dc.identifier.issn13-1982
dc.identifier.issue10tr
dc.identifier.pmid37895364en_US
dc.identifier.scopus2-s2.0-85192839141en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage1982tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14843
dc.identifier.volume13tr
dc.identifier.wosWOS:001094321500001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMDPItr
dc.relation.ispartofLifeen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/openAccesstr
dc.subjectazomethine grouptr
dc.subjectcytotoxicitytr
dc.subjectDNA repair genetr
dc.subjectgastric cancertr
dc.subjectgene expressiontr
dc.subjectmolecular dockingtr
dc.titleIn Silico and In Vitro Studies of Novel Azomethines on DNA Repair Genes in Gastric Cell Linesen_US
dc.typeArticleen_US

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