Molecular docking to investigate the inhibitory activity and the role of nitric oxide in anticonvulsant effects of vitamin D on pentylenetetrazole?induced epileptic seizures in rats

Basit Öğe Kaydı
dc.authorid0000-0001-9139-1884tr
dc.contributor.authorAkkaya, Recep
dc.contributor.authorAkkaya, Birnur
dc.contributor.authorBello, R. O.
dc.date.accessioned2024-02-27T11:56:33Z
dc.date.available2024-02-27T11:56:33Z
dc.date.issued2023tr
dc.departmentFen Fakültesitr
dc.description.abstract© 2022 Elsevier B.V.Despite the great breakthroughs in the field of epilepsy studies, the present drugs are ineffective in one-third of the patients, as well as without providing a definite treatment. There is accumulating evidence suggesting the role of vitamin D in epilepsy. The aim of this study was to investigate the effect of vitamin D on seizure formation, post-seizure cognitive functions, and the possible role of nitric oxide and oxidative stress in this effect, in the PTZ-induced seizure rat model. Sixty male Wistar albino rats were used in this study. The rats were randomly divided into 11 groups each containing 6 animals: Group 1: Control; Group 2: Saline+PTZ (45 mg/kg); Group 3: Vitamin D (1.5 mg/kg)+PTZ; Group 4: L-Arginine (500 mg/kg)+PTZ; Group 5: L-NAME (60 mg/kg)+PTZ; Group 6: 7-Nitroindazole (40 mg/kg); Group 7: Aminoguanidine (100 mg/kg)+PTZ; Group 8: Vitamin D+L- Arginine+PTZ; Group 9: Vitamin D+L-NAME+PTZ; Group 10: Vitamin D+7-Nitroindazole+PTZ; Group 11: Vitamin D+Aminoguanidine+PTZ. Animal behavior was evaluated with open field and passive avoidance tests. Nitric oxide (NO), total oxidative status (TOS), and total antioxidant status (TAS) levels in the cortex and hippocampus brain regions were determined by ELISA method. The treatment of vitamin D in combination with NO inhibitors has a positive effect on the PTZ-induced seizures and ameliorate post-seizure learning deficits. Moreover, the combination of vitamin D with NO inhibitors reduced oxidative stress and NO levels after seizures in the cortex and hippocampus. Also, we employed molecular docking and density functional theory analysis to investigate the inhibitory activity, reactivity, and stability of Vitamin D with other NO inhibitors. Vitamin D supplementation could be effective as a supportive treatment drug in epileptic patients, according to the findings.tr
dc.description.sponsorship...tr
dc.identifier.citation...tr
dc.identifier.doi10.1016/j.molstruc.2022.134246en_US
dc.identifier.endpage10tr
dc.identifier.issue1tr
dc.identifier.scopus2-s2.0-85140484430en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage1tr
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022286022018968?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14375
dc.identifier.volume1273tr
dc.identifier.wosWOS:000911581500004en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherJournal of Molecular Structuretr
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/openAccesstr
dc.subjectEpilepsy, Nitric oxide, Pentylenetetrazole, Rat, Vitamin D, DFT, Molecular dockingtr
dc.titleMolecular docking to investigate the inhibitory activity and the role of nitric oxide in anticonvulsant effects of vitamin D on pentylenetetrazole?induced epileptic seizures in ratsen_US
dc.typeArticleen_US

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