Did MALDI-TOF MS Increase Anaerobe Identification in the Microbiology Laboratory? A University Hospital Experience

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dc.contributor.authorTaşkin Kafa, Ayşe Hümeyra
dc.contributor.authorÇelik, Cem
dc.contributor.authorHasbek, Mürşit
dc.contributor.authorBakici, Mustafa Zahir
dc.date.accessioned2024-10-26T17:51:16Z
dc.date.available2024-10-26T17:51:16Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjective: This study aimed to investigate how the number of species identified in various clinical samples sent with the anaerobic culture request changed with Matrix Assisted Laser Desorption and Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS). Material and Methods: A total of 602 samples were included in the study, consisting of 419 different clinical samples sent from various polyclinics and services to the Microbiology Laboratory of Sivas Cumhuriyet University Medical Faculty with the anaerobic culture request, and 183 stool samples sent with the Clostridioides difficile request between 2015 and 2018. The results of the anaerobic culture requests within the mentioned years were analyzed retrospectively from the BD EpiCenter™ (Becton Dickin-son, USA) data analysis system. In the study, MALDI-TOF MS measurements were performed using a Bruker Microflex™ LT MALDI-TOF MS device and flexControl 3.0 software (Bruker Daltonics, USA), and MALDI Biotyper® 3.0 software and database were used for typing. Results: In the anaerobic cultures of 419 specimens sent from different clinical materials, 46 different anaerobic bacteria species were identified by MALDI-TOF MS method and 10 different anaerobic bacteria species were identified in stool samples. Among the identified anaerobic bacterial isolates, the most common genus was found to be Prevotella with 26.4%, followed by Cutibacterium acnes with 17.6%, Bacteroides with 12%, and Fusobac-terium with 8.8%, respectively. C. perfringens (47.3%) was identified as the most frequently identified species among 183 stool samples sent with the C. difficile request. Conclusion: When the results of our study were compared with the results of conventional methods, it was observed that there was an increase in the identification of anaerobic bacteria with MALDI-TOF MS system. We believe that the MALDI-TOF MS system can contribute to the identification of anaerobic bacteria. © 2020 by Türkiye Klinikleri.
dc.identifier.doi10.5336/medsci.2019-71905
dc.identifier.endpage202
dc.identifier.issn1300-0292
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85086664093
dc.identifier.scopusqualityQ4
dc.identifier.startpage196
dc.identifier.trdizinid388081
dc.identifier.urihttps://doi.org/10.5336/medsci.2019-71905
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/388081
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26125
dc.identifier.volume40
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkiye Klinikleri
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnaerobe; MALDI-TOF MS
dc.titleDid MALDI-TOF MS Increase Anaerobe Identification in the Microbiology Laboratory? A University Hospital Experience
dc.title.alternativeMALDI-TOF MS Mikrobiyoloji Laboratuvarında Anaerop Tanımlamalarını Artırdı mı? Bir Üniversite Hastanesi Deneyimi
dc.typeArticle

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