Development of sodium hydroxide-induced homogenous liquid-liquid extraction-effervescent assisted dispersive liquid-liquid microextraction based on deep eutectic solvents; Application in the extraction of phytosterols from cow cream samples

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dc.contributor.authorNemati, Mahboob
dc.contributor.authorTuzen, Mustafa
dc.contributor.authorAltunay, Nail
dc.contributor.authorFarajzdeh, Mir Ali
dc.contributor.authorAbdi, Fardin
dc.contributor.authorMogaddam, Mohammad Reza Afshar
dc.date.accessioned2024-10-26T18:10:53Z
dc.date.available2024-10-26T18:10:53Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn this study, sodium hydroxide-induced homogenous liquid-liquid extraction was combined with effervescent assisted-dispersive liquid-liquid microextraction and used for the extraction of phytosterols (lupeol, beta-sitosterol, stigmasterol, campesterol, and brassicasterol) from cream samples. Two types of deep eutectic solvents (watermiscible and water-immiscible solvents) were prepared and used in the extraction procedure. In this approach, firstly, the sample was dissolved in a mixture of n-hexane and tetrabutylammonium bromide: ethylene glycol deep eutectic solvent (as extraction/disperser solvent) and a homogenous solution was obtained. After that, a few microliters of sodium hydroxide solution (as fatty acid saponification and phase separation agent) was quickly injected into the mixture. By doing so, a three-phase system was formed. Then, the supernatant phase (tetrabutylammonium bromide: ethylene glycol deep eutectic solvent) was removed and mixed with tetrabutylammonium bromide: dichloroacetic acid: octanoic acid deep eutectic solvent. The obtained solution was applied in the microextraction step. To obtain effective, reliable, robust, accurate, and precise analytical results, the effective parameters for the presented method were investigated and optimized in detail. Detection limits, extraction recoveries, and enrichment factors were in the ranges of 0.06-0.26 mu g kg(-1), 86-93 %, and 215-232, respectively. Intra- and inter-day repeatabilities were investigated at a concentration of 1.5 mu g kg(-1) (each analyte) and they were in the ranges of 5.3-7.5 and 7.2-8.8 %, respectively. Finally, the presented method was easily used in determination of the phytosterols in cow milk cream samples.
dc.description.sponsorshipTabriz University of Medical Sciences [60046]
dc.description.sponsorshipThe authors would like to thank the Tabriz University of Medical Sciences for financial support under grant No. of 60046.
dc.identifier.doi10.1016/j.jfca.2021.104291
dc.identifier.issn0889-1575
dc.identifier.issn1096-0481
dc.identifier.scopus2-s2.0-85119937103
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jfca.2021.104291
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30430
dc.identifier.volume106
dc.identifier.wosWOS:000803854200027
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofJournal of Food Composition and Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHomogenous liquid-liquid extraction
dc.subjectCow milk cream
dc.subjectMass spectrometry
dc.subjectDispersive liquid-liquid
dc.subjectMicroextraction
dc.subjectPhytosterol
dc.subjectGas chromatography
dc.titleDevelopment of sodium hydroxide-induced homogenous liquid-liquid extraction-effervescent assisted dispersive liquid-liquid microextraction based on deep eutectic solvents; Application in the extraction of phytosterols from cow cream samples
dc.typeArticle

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