Vitamin D increases the efficacy of cisplatin on bladder cancer cell lines

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dc.authoridOZGEN, OZGE/0000-0002-1859-3962
dc.contributor.authorOzgen, Ozge
dc.contributor.authorEroglu, Gunes Ozen
dc.contributor.authorKucukhuseyin, Ozlem
dc.contributor.authorAkdeniz, Nilgun
dc.contributor.authorHepokur, Ceylan
dc.contributor.authorKuruca, Serap
dc.contributor.authorYaylim, Ilhan
dc.date.accessioned2024-10-26T18:07:25Z
dc.date.available2024-10-26T18:07:25Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground 1,25(OH)2D3(Calcitriol), which is a broad regulatory molecule, plays a role in changing the efficacy of chemotherapeutic drugs. Cisplatin is one of a current standard chemotherapy regimen for bladder cancer. Increasing the effectiveness of the treatment and reducing the side effects to chemotherapeutics are of great importance in bladder cancer. We aimed to investigate the effect of the combination of cisplatin and calcitriol in order to create a possible advantage in treatment of bladder cancer. Methods T24, ECV-304 and HUVEC cell lines were treated with calcitriol and cisplatin individually and in combination. Dose determination and combination treatments of calcitriol and cisplatin were evaluated using the MTT assay for cytotoxicity analysis on the cells. Annexin V-PI staining method was used for apoptosis determination by flow cytometry. Also the P-gp expression levels were determined by flow cytometry. Results The combination treatment increased the anti-proliferative efficacy compared to the efficacy in cisplatin alone in T24 cells and reduced the cytotoxicity in the HUVEC healthy cells compared to cisplatin alone. Combination treatment achieved significantly higher apoptosis rate in T24 cells compared with the rates in treatment of cisplatin alone. However apoptosis decreased in HUVEC cell line. P-gp ratios were increased in HUVEC and decreased in T24 cells with combination treatment compared to the numbers in the control cells. The rate of apoptosis and P-gp levels showed no significant change in ECV-304 cells. Conclusion Our study revealed that the combination of calcitriol and cisplatin allows the use of cisplatin at lower doses in T24 bladder cancer cell line.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University [TDK-2017-23523]
dc.description.sponsorshipThe present study was supported by the grant of the Scientific Research Projects Coordination Unit of Istanbul University (Doctorate Project no: TDK-2017-23523). We thank to Abdullah Ylmaz fort he flow cytometric analysis. Also we thank to Assoc Prof Dr Nuray Varol for the material supplement.
dc.identifier.doi10.1007/s11033-022-08044-2
dc.identifier.endpage706
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1
dc.identifier.pmid36370297
dc.identifier.scopus2-s2.0-85141679907
dc.identifier.scopusqualityQ2
dc.identifier.startpage697
dc.identifier.urihttps://doi.org/10.1007/s11033-022-08044-2
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29483
dc.identifier.volume50
dc.identifier.wosWOS:000883156900006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBladder cancer
dc.subjectT24 cell line
dc.subjectCalcitriol
dc.subjectCisplatin
dc.subjectVitamin D
dc.subjectP-gp
dc.subjectApoptosis
dc.titleVitamin D increases the efficacy of cisplatin on bladder cancer cell lines
dc.typeArticle

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