The relationship between lymphocyte DNA damage, coronary artery disease, and blood trace elements

Basit Öğe Kaydı
dc.authoridDural, Emrah/0000-0002-9320-8008
dc.contributor.authorErdogu, Ismail
dc.contributor.authorDural, Emrah
dc.contributor.authorAltundag, Huseyin
dc.contributor.authorOymak, Tulay
dc.contributor.authorSuzen, Halit Sinan
dc.date.accessioned2024-10-26T18:09:38Z
dc.date.available2024-10-26T18:09:38Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractSomatic DNA damage and causative factors (occupational exposures, foods, habits, etc.) are thought to contribute to the pathogenesis of atherosclerosis, although knowledge about their role in coronary artery disease (CAD) is still insufficient. This study aimed to determine the effects of lymphocyte-DNA damage and blood trace element concentrations on CAD. The single-cell alkaline comet was used in the measuring of the lymphocyte DNA damage in blood samples obtained from patients (n = 99) whose CAD grade was determined by the syntax score while the angiographic intervention was carried out. Blood trace element (n = 14) concentrations were monitored by the inductively coupled plasma-optical emission spectroscopy (ICP-OES) after microwave digestion. The relationship between the DNA damage frequencies of the participants and their syntax scores, blood trace element concentrations, and other demographic and clinic parameters were statistically analyzed. Significant correlations were detected between comet data and syntax score (r = 0.858, P < .001), age (r = 0.337, P < .001), blood-urea (r = 0.360, P < .001), creatinine (r = 0.388, P < .001), HbA1c (0.218, P < .05), ECG-QRS time (r = 0.286, P < .01), ECHO-EF (r = -0.377, P < .001), and platelet (r = -0.222, P < .05). The DNA damage frequencies of the groups formed according to their CAD scores were significantly different from the control group (P < .001) and also each other (P <= .01). Comet frequencies and CAD grades were found to be correlated with aging (P < .05). DNA damage frequency and syntax score values were significantly (P < .05) higher in males compared to females. Syntax scores were correlated with aging (r = 0.348, P < .01), ECHO-EF (r = 0.374, P < .001), blood-urea (r = 0.398, P < .001), creatinine (r = 0.433, P < .001), glucose (0.218, P < .05), and HbA1c (r = 0.200, P < .05). Significant correlations were observed between trace elements and demographic values, blood parameters, diseases, angio parameters, ECHO, and ECG parameters. It was observed that the concentrations of trace elements detected in the blood were 93.4% correlated with each other. Lymphocyte DNA damage is a strong biomarker for the atherosclerotic indicator of CAD. Aging is an effective factor both in the DNA damage frequency and CAD risk index. Creatinine and urea are factors that have the power to change the CAD risk index and DNA damage frequency. The higher DNA damage and CAD risk were monitored in males compared to females. The relationship between some biomarkers and blood trace element concentrations showed that further studies are needed to more accurately evaluate the relationship between trace elements, DNA damage frequencies, and CAD.
dc.description.sponsorshipScientific Research Project Coordination Office of Cumhuriyet University, Sivas-Turkey [ECZ080]
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Office of Cumhuriyet University, Sivas-Turkey [ECZ080, 2020].
dc.identifier.doi10.1093/mutage/gead030
dc.identifier.endpage55
dc.identifier.issn0267-8357
dc.identifier.issn1464-3804
dc.identifier.issue1
dc.identifier.pmid37818856
dc.identifier.scopus2-s2.0-85184660996
dc.identifier.scopusqualityQ2
dc.identifier.startpage43
dc.identifier.urihttps://doi.org/10.1093/mutage/gead030
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30185
dc.identifier.volume39
dc.identifier.wosWOS:001086974900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofMutagenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectlymphocyte DNA damage
dc.subjectComet assay
dc.subjectblood trace elements
dc.subjectICP-OES
dc.subjectcoronary artery disease
dc.subjectSYNTAX score
dc.titleThe relationship between lymphocyte DNA damage, coronary artery disease, and blood trace elements
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksyonu
Scopus İndeksli Yayınlar Koleksiyonu