Do antidiabetic drugs prevent the transformation of Acanthamoeba trophozoite into cyst form?

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dc.contributor.authorOzpinar, Necati
dc.contributor.authorKaraman, Ulku
dc.contributor.authorOzpinar, Hulya
dc.contributor.authorDag, Seker
dc.date.accessioned2024-10-26T18:09:01Z
dc.date.available2024-10-26T18:09:01Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study examines the effects of three different drugs with metformin, acarbose and pioglitazone active ingredients used for antidiabetic purposes on Acanthamoeba cysts and trophozoites. Cultures of A. castellanii trophozoites and cysts were prepared to test the anti-amoebic activity of metformin, acarbose and pioglitazone. Cultures were then prepared for A. castellanii cyst and trophozoite forms and parasites were exposed to different concentrations (0.750 mg/mL, 0.375 mg/mL, 0.186 mg/mL and 0.093 mg/mL) of metformin, acarbose and pioglitazone. As a result of the study, the reproductive potential suppressive effects and conversion from trophozoite form to cyst form of all three substances on A. castellanii trophozoites and cysts were determined. Parasites were counted at 12, 24 and 48 hours in the cell counter after staining with trypan blue. In comparison of the effects of metformin, acarbose and pioglitazone used in the study on A. castellanii trophozoites and cysts, it was observed that all three substances were statistically effective against cysts and trophozoites at a concentration of 0.750 mg/mL. Furthermore, it was determined that all concentrations of the three active substances included in the study significantly decreased the rate of cyst formation even at the end of the 7th day. In this context, it was determined that all three substances have amebicidal effects, and they significantly inhibit the transformation of A. castellanii trophozoites to cyst form. It is thought that these active substances, which are currently used as anti-diabetic, can be used in combination with other drugs in A. castellanii infections based on our study findings.
dc.identifier.doi10.1080/20477724.2022.2151859
dc.identifier.endpage680
dc.identifier.issn2047-7724
dc.identifier.issn2047-7732
dc.identifier.issue7
dc.identifier.pmid36436006
dc.identifier.scopus2-s2.0-85142790590
dc.identifier.scopusqualityQ2
dc.identifier.startpage674
dc.identifier.urihttps://doi.org/10.1080/20477724.2022.2151859
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29901
dc.identifier.volume117
dc.identifier.wosWOS:000890388100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofPathogens and Global Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcanthamoeba
dc.subjectamoebicidal activity
dc.subjectantidiabetic drugs
dc.subjectmetformin
dc.subjectacarbose
dc.subjectpioglitazone
dc.titleDo antidiabetic drugs prevent the transformation of Acanthamoeba trophozoite into cyst form?
dc.typeArticle

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