From parasitic life to health-promoting applications- A versatile goldmine discovered in nature's secret treasure chest: Orobanche nana

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dc.authoridBerisha, Avni/0000-0002-3876-1345
dc.contributor.authorKurt-Celep, Inci
dc.contributor.authorYagi, Sakina
dc.contributor.authorDall'Acqua, Stefano
dc.contributor.authorSut, Stefania
dc.contributor.authorCelep, Engin
dc.contributor.authorKaya, Savas
dc.contributor.authorBerisha, Avni
dc.date.accessioned2025-05-04T16:47:12Z
dc.date.available2025-05-04T16:47:12Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractStudies evaluating the phytoconstituents and therapeutic potential of species belonging to Orobanche (Family Orobanchaceae) are rather limited. The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of the aerial parts of Orobanche nana Noe ex Rchb. Extracts were prepared by sequential maceration in dichloromethane, ethyl acetate and ethanol and finally an aqueous extraction by infusion were performed. NMR and LC-DAD-MS analysis allowed the identification of different phytoconstituents including the characteristic compounds of Orobanche like verbascoside, poliumoside, orobanone and orobanchoside. The ethanol extract displayed the highest total phenolic (115.63 mg GAE/g) and flavonoids (16.53 mg RE/g) contents, antiradical (DPPH = 623.70 mg TE/g; ATBS = 843.50 mg TE/g) and ions reducing (Cu++ = 725.39; Fe+++ = 617.70) properties while the aqueous extract exerted the best chelating power (19.54 mg EDTAE/g). It was determined that EtOH and Water extracts applied to HepG2 cells decreased the levels of anti-apoptotic proteins p-NF kappa B, BCL-2 and BCL-XL, while increasing the levels of apoptotic proteins BAX and p-53, thus inhibiting cell survival. In addition, Orobanche nana played an active role in the rearrangement of overexpress MMPs that cause disruption of ECM homeostasis. We employed Network Pharmacology to investigate the mechanisms through which Orobanche nana's compounds impact Kidney and Liver diseases. These findings suggested that O. nana could be a promising source of bioactive molecules with potential therapeutic applications.
dc.identifier.doi10.1016/j.fbio.2024.105296
dc.identifier.issn2212-4292
dc.identifier.issn2212-4306
dc.identifier.scopus2-s2.0-85206801629
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.fbio.2024.105296
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35530
dc.identifier.volume62
dc.identifier.wosWOS:001342477900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofFood Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectOrobanche nana
dc.subjectPhytoconstituents
dc.subjectantioxidant
dc.subjectEnzyme inhibition
dc.subjectCytotoxicity
dc.titleFrom parasitic life to health-promoting applications- A versatile goldmine discovered in nature's secret treasure chest: Orobanche nana
dc.typeArticle

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