IgG purification by bentonite-acrylamide-histidine microcomposite

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dc.contributor.authorAkkaya, Birnur
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:03:58Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:03:58Z
dc.date.issued2012
dc.departmentCumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkeyen_US
dc.description.abstractIn this work, a new microcomposite composed of bentonite, acrylamide and histidine, as a pseudospecific ligand, was synthesized by bulk polymerization. The aim of this study was to improve IgG adsorption capacity of bentonite by incorporating histidine. The surface areas of the bentonite and bentonite-acrylamide-histidine microcomposites were 33.4 and 1.42 m(2)/g, respectively. The amount of histidine was found to be 50 mu mol/g bentonite via elemental analysis. Adsorption capacity was at the value of 100 mg/g from aqueous solution while adsorption capacity was 108 mg/g from human plasma with a purity of 90%. IgG biomolecules were able to be adsorbed and desorbed five times by using the same microcomposites without significant loss in their adsorption capacity. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipCumhuriyet University (CUBAP) [F-321]en_US
dc.description.sponsorshipThis work was supported by The Research Fund of Cumhuriyet University (CUBAP, Project no: F-321). The author is also grateful to Dr. Recep Akkaya for providing valuable experimental studies.en_US
dc.identifier.doi10.1016/j.colsurfb.2011.11.033en_US
dc.identifier.endpage155en_US
dc.identifier.issn0927-7765
dc.identifier.pmid22197734en_US
dc.identifier.scopus2-s2.0-84856079379en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage151en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfb.2011.11.033
dc.identifier.urihttps://hdl.handle.net/20.500.12418/9203
dc.identifier.volume92en_US
dc.identifier.wosWOS:000300859000021en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofCOLLOIDS AND SURFACES B-BIOINTERFACESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcrylamideen_US
dc.subjectAffinity chromatographyen_US
dc.subjectBentoniteen_US
dc.subjectHistidineen_US
dc.subjectMicrocompositeen_US
dc.subjectIgG purificationen_US
dc.titleIgG purification by bentonite-acrylamide-histidine microcompositeen_US
dc.typeArticleen_US

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