A PLK1 inhibitor, RO3280, suppresses proliferation of SNU-16 gastric cancer cells via apoptosis

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dc.contributor.authorYulak, Fatih
dc.contributor.authorKeskin, Zekeriya
dc.date.accessioned2024-10-26T18:05:28Z
dc.date.available2024-10-26T18:05:28Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study examines the anti -tumor, pro-apoptotic, and genotoxic effects of polo -like kinase 1 (PLK1) inhibitor RO3280 on the gastric cancer cell line SNU16. Using the XTT assay, the antiproliferative effect of the compound was identified and showed a significant antiproliferative effect (p<0.01) with the IC50 value of 9.64 M for 24 hours. RO3280 considerably enhanced the expression of Bax and cleaved caspase-3 (p<0.01) and dramatically lowered the BCL-2 level (p<0.01), demonstrating an apoptotic effect. It greatly raised the expression of 8-oxo-dG, a DNA damage marker, and cleaved PARP, a degraded version of the PARP enzyme involved in DNA repair (p<0.05 and p<0.01, respectively). In addition, flow cytometric studies for annexin V and caspase3/7 revealed that RO3280 markedly improved the apoptotic cell profile (p<0.01). It reduced the mitochondrial membrane potential and DNA damage (p<0.01). These findings suggest that RO3280 has an antitumor impact by damaging DNA and inducing apoptosis in SNU-16 gastric cancer cells.
dc.identifier.doi10.3329/bjp.v19i2.71704
dc.identifier.issn1991-007X
dc.identifier.issn1991-0088
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85196909999
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.3329/bjp.v19i2.71704
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29022
dc.identifier.volume19
dc.identifier.wosWOS:001252660500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherBangladesh Pharmacological Soc
dc.relation.ispartofBangladesh Journal of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleA PLK1 inhibitor, RO3280, suppresses proliferation of SNU-16 gastric cancer cells via apoptosis
dc.typeArticle

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