In vitro anticancer, antioxidant and enzyme inhibitory potentials of endemic Cephalaria elazigensis var. purpurea with in silico studies

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dc.authoridBehcet, Lutfi/0000-0001-8334-7816
dc.authoridTaslimi, Parham/0000-0002-3171-0633
dc.authoridTASKIN-TOK, Tugba/0000-0002-0064-8400
dc.authoridKIRICI, Mahinur/0000-0003-4642-7387
dc.authoridErdogan, Mehmet Kadir/0000-0002-1579-5737
dc.authoridTUZUN, BURAK/0000-0002-0420-2043
dc.authoridgecibesler, ibrahim/0000-0002-4473-2671
dc.contributor.authorErdogan, Mehmet Kadir
dc.contributor.authorGundogdu, Ramazan
dc.contributor.authorYapar, Yakup
dc.contributor.authorGecibesler, Ibrahim Halil
dc.contributor.authorKirici, Mahinur
dc.contributor.authorBehcet, Lutfi
dc.contributor.authorTuzun, Burak
dc.date.accessioned2024-10-26T18:09:37Z
dc.date.available2024-10-26T18:09:37Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn this study, the therapeutic potential and phytochemical composition of ethanolic extract of Cephalaria elazigensis var. purpurea (CE), an endemic species, were investigated. For this purpose, the antiproliferative effect of CE on the MCF-7 human breast cancer cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and its effectiveness on colony formation and cell migration was analyzed with clonogenic assay and wound healing assay, respectively. In addition, the cell death detection ELISA (CDDE) assay was conducted to determine the pro-apoptotic capacity of CE. The IC50 value of the CE was determined as 324.2 +/- 14.7 mu g/mL. Furthermore, upon 1000 mu g/mL CE treatment, there was 4.96-fold increase in the population of cells undergoing apoptosis compared to the untreated control cells. The antioxidant activity tests were performed by DPPH free radical, ABTS cation radical, ferric-ion reducing power (FRAP) and ferrous-ion chelating power (FCAP) assays. Antioxidant activity values for the DPPH, ABTS and FRAP assays were found to be 125.6 +/- 6.3, 34.09 +/- 0.1 and 123.4 +/- 4.2 mu mol TE/mg DE, respectively. We further determined the effect of CE ethanolic extract against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) enzymes. CE plays an effective inhibitory role in AChE and BuChE (AChE: IC50: 10.54 mu g/mL, BuChE: IC50: 6.84 mu g/mL) respectively. Further, molecular docking stuy was conducted to understand the nature of the all compound against AChE an BChE. It is revealed that alpha-Linolenic acid shows lowest binding energy (-7.90 kcal/mol) towards AChE, on the other side, Linoleic acid shows good binding affinity (-7.40 kcal/mol) for BChE.Communicated by Ramaswamy H. Sarma
dc.identifier.doi10.1080/07391102.2022.2163700
dc.identifier.endpage11844
dc.identifier.issn0739-1102
dc.identifier.issn1538-0254
dc.identifier.issue21
dc.identifier.pmid36637391
dc.identifier.scopus2-s2.0-85146755000
dc.identifier.scopusqualityQ2
dc.identifier.startpage11832
dc.identifier.urihttps://doi.org/10.1080/07391102.2022.2163700
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30176
dc.identifier.volume41
dc.identifier.wosWOS:000913534500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Biomolecular Structure & Dynamics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCephalaria elazigensis var
dc.subjectpurpurea
dc.subjectbreast cancer
dc.subjectapoptosis
dc.subjectAlzheimer diseases
dc.subjectantioxidant
dc.titleIn vitro anticancer, antioxidant and enzyme inhibitory potentials of endemic Cephalaria elazigensis var. purpurea with in silico studies
dc.typeArticle

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