Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models

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dc.authoridDjordjic Crnogorac, Marija/0000-0002-5820-7202
dc.authoridAliyazicioglu, Yuksel/0000-0001-9474-4307
dc.authoridMatic, Ivana/0000-0001-7364-2619
dc.authoridDamjanovic, Ana/0000-0002-0781-8433
dc.authoridStanojkovic, Tatjana/0000-0001-9178-9200
dc.authoridPetrovic, Nina/0000-0003-2503-1228
dc.contributor.authorMatic, Ivana Z.
dc.contributor.authorErgun, Sercan
dc.contributor.authorDordic Crnogorac, Marija
dc.contributor.authorMisir, Sema
dc.contributor.authorAliyazicioglu, Yuksel
dc.contributor.authorDamjanovic, Ana
dc.contributor.authorDzudzevic-Cancar, Hurija
dc.date.accessioned2024-10-26T18:11:30Z
dc.date.available2024-10-26T18:11:30Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractSix extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2-6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2-6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts.
dc.description.sponsorshipMinistry of Education, Science and Technological Development of the Republic of Serbia [451-03-9/2021-14/200043]; Scientific Research Projects Unit of Ordu University [A-1823]
dc.description.sponsorshipThe authors are grateful to the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 451-03-9/2021-14/200043) and Scientific Research Projects Unit of Ordu University (Project A-1823) for the financial support.
dc.identifier.doi10.1007/s10616-021-00464-5
dc.identifier.endpage389
dc.identifier.issn0920-9069
dc.identifier.issn1573-0778
dc.identifier.issue3
dc.identifier.pmid34149173
dc.identifier.scopus2-s2.0-85103594364
dc.identifier.scopusqualityQ3
dc.identifier.startpage373
dc.identifier.urihttps://doi.org/10.1007/s10616-021-00464-5
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30696
dc.identifier.volume73
dc.identifier.wosWOS:000635859500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCytotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHypericum perforatum
dc.subjectCytotoxic activity
dc.subjectMatrix-metalloproteinase
dc.subjectVascular endothelial growth factor
dc.subjectmiR128
dc.subject193a-5p
dc.subject335
dc.subject3D HeLa spheroids
dc.titleCytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
dc.typeArticle

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