Optimization and application of ultrasound-assisted sugar based deep eutectic solvent dispersive liquid-liquid microextraction for the determination and extraction of aflatoxin M1 in milk samples

Basit Öğe Kaydı
dc.contributor.authorGursoy, Nevcihan
dc.contributor.authorSirtbasi, Busra
dc.contributor.authorSimsek, Selcuk
dc.contributor.authorElik, Adil
dc.contributor.authorAltunay, Nail
dc.date.accessioned2024-10-26T18:02:31Z
dc.date.available2024-10-26T18:02:31Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study presents an ultrasound-assisted sugar based deep eutectic solvent dispersive liquid-liquid micro-extraction procedure (UA sugar-DES DLLME) for the extraction of aflatoxin M-1 (AFM(1)) in milk samples prior to its determination by UV-VIS spectrophotometer. Also, in this approach, different sugar based deep eutectic solvents were prepared and their ability in extraction and separation of the AFM(1) from the samples were investigated. The UA sugar-DES DLLME procedure consists of three steps. In the first step, pyrogallol and sugar based-DES solutions were added to the sample solution at pH 3.8. In the second step, ultrasound was applied to the solution obtained and the AFM(1) were extracted into the UA sugar based-DES phase. In the final step, the resulting mixture was centrifuged and the sediment phase was separated from the mixture. After the micro-extraction, the sediment phase was analyzed at 279 nm by UV-VIS spectrophotometer. In order to obtain reli-able, accurate and precise analytical results, the effective microextraction parameters were investigated and optimized using response surface methodology. Under optimum conditions, detection limit, extraction recovery, and enrichment factor were 6.1 ng L-1, 91 +/- 3-107 +/- 6%, and 328, respectively. Intraday and inter-day studies were performed at concentrations of 50, 100 and 200 ng L-1 of the AFM(1) for the precision of the UA sugar-DES DLLME procedure. The intraday and inter-day precision results ranged from 3.3 to 5.1% and 4.1-6.2%, respectively. Also, it was successfully used for determination of the selected milks.
dc.identifier.doi10.1016/j.microc.2021.106974
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85118892154
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2021.106974
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28175
dc.identifier.volume172
dc.identifier.wosWOS:000719818300007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAflatoxinM(1)
dc.subjectMilks
dc.subjectELISA analysis
dc.subjectChemometric optimization
dc.subjectDeep eutectic solvent
dc.titleOptimization and application of ultrasound-assisted sugar based deep eutectic solvent dispersive liquid-liquid microextraction for the determination and extraction of aflatoxin M1 in milk samples
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu