Effects of probiotics on GABA/glutamate and oxidative stress in PTZ-induced acute seizure model in rats

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dc.contributor.authorCiltas, Arzuhan Cetindag
dc.contributor.authorToy, Cemal Erdem
dc.contributor.authorGunes, Handan
dc.contributor.authorYaprak, Meryem
dc.date.accessioned2024-10-26T18:04:06Z
dc.date.available2024-10-26T18:04:06Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractStudies conducted in recent years have indicated a relationship between epilepsy and gut microbiota. Ion channels, excitatory/inhibitory balance and regulatory systems play a role in the pathophysiology of epilepsy. In addition, gut dysbiosis is also involved in the pathophysiology of epilepsy. This research investigated the impacts of probiotic mixture on epileptic seizures, Gamma aminobutyric acid (GABA), glutamate, and TAS and TOS levels in hippocampal tissue in the PTZ-induced acute seizure model in rats. Four groups were formed with male Wistar albino rats. The first and second groups were given 1 ml/day saline solution, and the other groups were given 0.05 mg/1 ml/day vehicle or 109cfu/1 ml/day probiotic supplementation, respectively via gavage for 21 days. A single-dose PTZ (45 mg/kg) was administered to induce seizure. The stages of seizure were analyzed according to the Racine scale. While ELISA was used to determine GABA and glutamate levels in the hippocampus, an automated colorimetric method was utilized to measure oxidant/antioxidant biomarkers. It was found that by delaying the first myoclonic jerk (FMJ), and the onset of the generalized tonic-clonic seizures, the probiotic mixture demonstrated anticonvulsant effects against seizures. The probiotic mixture was found to increase the inhibitory neurotransmitter GABA. It was also found to decrease TOS levels and increase TAS concentration. The findings of this study showed that probiotic mixture reduced oxidative stress with its positive effects against PTZinduced epileptic seizures. Further studies are needed to reveal potentially related mechanisms.
dc.description.sponsorship2209-A University Students Research Projects Support Program [1919B012102203]
dc.description.sponsorshipThis work was supported by the 2209-A University Students Research Projects Support Program with the project code 1919B012102203.
dc.identifier.doi10.1016/j.eplepsyres.2023.107190
dc.identifier.issn0920-1211
dc.identifier.issn1872-6844
dc.identifier.pmid37473590
dc.identifier.scopus2-s2.0-85166753333
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.eplepsyres.2023.107190
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28750
dc.identifier.volume195
dc.identifier.wosWOS:001046196500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofEpilepsy Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpilepsy
dc.subjectMicrobiota
dc.subjectProbiotic
dc.subjectGABA
dc.subjectGlutamate
dc.subjectOxidative stress
dc.subjectRat
dc.titleEffects of probiotics on GABA/glutamate and oxidative stress in PTZ-induced acute seizure model in rats
dc.typeArticle

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