Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method

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dc.authoridDural, Emrah/0000-0002-9320-8008
dc.contributor.authorDural, Emrah
dc.contributor.authorKaya, Beyza Nur
dc.date.accessioned2024-10-26T18:00:34Z
dc.date.available2024-10-26T18:00:34Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground and Aims: Diazepam is accepted as a safer drug to medicate in many serious cases, acting as an anticonvulsant, an anxiolytic and a treatment for many types of poisoning. Monitoring it is important in achieving successful treatment and reducing the risk of toxic effects. In this study, it is aimed to develop and validate a sensitive, repeatable, and reliable method based on high-performance liquid chromatographic analysis for the determination in human plasma. Methods: Separation was carried out using a reverse-phase C18 column (4.0 mm x 150 mm, 3 mu m) at 30 degrees C. The solution was prepared with a 10 mM phosphate buffer and acetonitrile (1:1, v/v) was employed as a mobile phase at the isocratic flow with 0.5 mL/min rate. Quantification was applied at 230 nm. A solid-phase extraction method was established and optimized, which was then used in the preparation of the plasma (0.5 mL) samples to the analysis. Results: The method was found to be linear (r(2) = 0.9805) between 100 and 1200 ng/mL. The analysis run was <= 12 min. Intraday and inter-day accuracy were found between -5.78 and 5.93 and precision was <= 1.82%. The limit of detection and quantification were calculated as 20.42 and 61.86 ng/mL, respectively. Recovery was found between the range of 95.12% and 106.83%. The method was determined to be robust according to changes in UV, mobile phase organic solvent content, mobile phase pH, column temperature, and operator. Conclusion: This simple, sensitive and reliable method is suggested for accredited-reference laboratories working on the therapeutic drug monitorization and/or overdose-toxicological quantified analysis of diazepam in human plasma.
dc.identifier.doi10.26650/IstanbulJPharm.2022.877867
dc.identifier.endpage46
dc.identifier.issn2587-2087
dc.identifier.issue1
dc.identifier.startpage37
dc.identifier.trdizinid1126961
dc.identifier.urihttps://doi.org/10.26650/IstanbulJPharm.2022.877867
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1126961
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27757
dc.identifier.volume52
dc.identifier.wosWOS:000793925000006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherIstanbul Univ, Fac Pharmacy
dc.relation.ispartofIstanbul Journal of Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiazepam
dc.subjectSolid-phase extraction
dc.subjectMethod validation
dc.subjectHigh-performance liquid chromatography-ultraviolet detection
dc.titleDetermination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method
dc.typeArticle

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