Death receptor-dependent apoptosis and cell cycle delay induced by bioymifi in human cervical cancer cells

Basit Öğe Kaydı
dc.authoridyulak, fatih/0000-0003-3708-6752
dc.contributor.authorAltun, Ahmet
dc.contributor.authorInan, Zeynep Deniz Sahin
dc.contributor.authorYulak, Fatih
dc.date.accessioned2024-10-26T18:02:43Z
dc.date.available2024-10-26T18:02:43Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn chemotherapy applied against cervical cancer, non-specific cytotoxicity and drug resistance that develops over time are trying to be overcome. Therefore, the development of effective and innovative chemotherapeutic drugs for the treatment is among the priority issues in the medical field. The anticancer activity of the Bioymifi, which can activate apoptosis by inducing DR-5 clustering and aggregation against the human cervical cancer cell line, was investigated in the current study. The cytotoxic activity of Bioymifi on the HeLa cell line was identified using XTT assay. The pathway of the cell death mechanism was analyzed through the cell cycle and Annexin V assays by the flow cytometry. DAPI staining assay was applied under fluorescence microscopy to examine the nuclear morphology. Bioymifi appeared to have a remarkable IC50 value (11.75??M) against HeLa cells. The cell cycle analysis demonstrated the increase of Bioymifi cured HeLa cells in the S phase. And also, 11.75??M of Bioymifi caused a significantly higher apoptotic effect compared to control. In addition, in vitro immunofluorescence experiments of this study represented that Bioymifi reduced Ki-67 localization in HeLa cells. Bioymifi has significantly anticancer actions in Human cervix cancer in vitro and can be combined with standard treatment.
dc.identifier.doi10.36721/PJPS.2022.35.4.REG.1031-1036.1
dc.identifier.endpage1036
dc.identifier.issn1011-601X
dc.identifier.issue4
dc.identifier.pmid36008899
dc.identifier.scopus2-s2.0-85136567778
dc.identifier.scopusqualityQ3
dc.identifier.startpage1031
dc.identifier.urihttps://doi.org/10.36721/PJPS.2022.35.4.REG.1031-1036.1
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28311
dc.identifier.volume35
dc.identifier.wosWOS:000818984200009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherUniv Karachi
dc.relation.ispartofPakistan Journal of Pharmaceutical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectbioymifi
dc.subjectcell cycle
dc.subjectcervical cancer
dc.subjectdeath receptor
dc.titleDeath receptor-dependent apoptosis and cell cycle delay induced by bioymifi in human cervical cancer cells
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksyonu
Scopus İndeksli Yayınlar Koleksiyonu