Genome-wide profiling of the expression of serum derived exosomal circRNAs in patients with hepatic alveolar echinococcosis

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dc.authoridozdemir, selcuk/0000-0001-7539-0523
dc.authoridozkaraca, mustafa/0000-0002-6359-6249
dc.contributor.authorOzdemir, Selcuk
dc.contributor.authorAksungur, Nurhak
dc.contributor.authorAltundas, Necip
dc.contributor.authorKara, Salih
dc.contributor.authorKorkut, Ercan
dc.contributor.authorOzkaraca, Mustafa
dc.contributor.authorMendil, Ali Sefa
dc.date.accessioned2024-10-26T18:10:56Z
dc.date.available2024-10-26T18:10:56Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThe patients with hepatic alveolar echinococcosis is poorly detected due to invasive and slow growth. Thus, early diagnosis of hepatic alveolar echinococcosis is so important for patients. Circular RNAs are crucial types of the non-coding RNA. Recent studies have provided serum-derived exosomal circRNAs as potential biomarkers for detection of various diseases. The clinical importance of exosomal circRNAs in hepatic alveolar echinococcosis have never been explored before. Here, we investigated the serum-derived exosomal circRNAs in the diagnosis of hepatic alveolar echinococcosis. Firstly, High-throughput Sequencing was performed using 9 hepatic alveolar echinococcosis and 9 control samples to detect hepatic alveolar echinococcosis related circRNAs. Afterwards, bioinformatic analyzes were performed to identify differentially expressed circRNAs and pathway analyzes were performed. Finally, validation of the determined circRNAs was performed using RT-PCR. The sequencing data indicated that 59 differentially expressed circRNAs; 31 up-regulated and 28 down-regulated circRNA in hepatic alveolar echinococcosis patients. The top 5 up-regulated and down-regulated circRNAs were selected for vali-dation by RT-qPCR assay. As a result of the verification, circRNAs that were significantly up-and down-regulated showed an expression profile consistent with the results obtained. Importantly, our findings suggested that identified exosomal circRNAs could be a potential biomarker for the detection of hepatic alveolar echinococcosis serum and may help to understand the pathogenesis of hepatic alveolar echinococcosis.
dc.identifier.doi10.1016/j.gene.2021.146161
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.pmid34995736
dc.identifier.scopus2-s2.0-85122519934
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.gene.2021.146161
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30450
dc.identifier.volume814
dc.identifier.wosWOS:000742709500008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofGene
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHepatic alveolar echinococcosis
dc.subjectCircRNA
dc.subjectRNA-seq
dc.subjectBiomarker
dc.subjectDiagnosis
dc.titleGenome-wide profiling of the expression of serum derived exosomal circRNAs in patients with hepatic alveolar echinococcosis
dc.typeArticle

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