Genotyping by Sequencing of Acanthamoeba and Naegleria Isolates from the Thermal Pool Distributed Throughout Turkey

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dc.authoridIlter, Huseyin/0000-0002-4452-8902
dc.authoridDegerli, Serpil/0000-0003-2229-1737
dc.authoridDEGERLI, NACI/0000-0003-0737-7014
dc.authoridCAMUR, DERYA/0000-0002-2970-674X
dc.contributor.authorDegerli, Serpil
dc.contributor.authorDegerli, Naci
dc.contributor.authorCamur, Derya
dc.contributor.authorDogan, Ozgul
dc.contributor.authorIlter, Huseyin
dc.date.accessioned2024-10-26T18:09:37Z
dc.date.available2024-10-26T18:09:37Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractPurpose The main goal of this study was genotyping of free-living parasites and sub-grouping of pathogenic or non-pathogenic amebae obtained from Turkey's thermal springs. In so doing, distribution and abundance of possible pathogenic or causative strain for humans, which are caused by Acanthamoeba and Naegleria strains, would be elaborated. The number of extensive studies on the general occurrence and distribution of parasitic strains is very high worldwide, but there has been a paucity of information with regard to Turkey. Methods From a total of 434 obtained thermal pool samples, free-living amebas were isolated from 148 water samples using the non-nutrient agar (NNA) culture method. Subsequently, the cultivated samples were used for DNA isolation; then 102 obtained DNA samples were subjected to PCR amplification using various primers for samples of genera Acanthamoeba and Naegleria. Ultimately, estimation of genotype or subtype was evaluated by sequencing. Results About 29 samples that belong to Acanthamoeba and Naegleria were estimated from a total of 102 amplified PCR samples. These eukaryotic PCR products which have Acanthamoeba genus appearance, generated 26 subtypes and 3 Naegleria samples. Among the 26 Acanthamoeba genotypes, 22 aligned sequences were matched with various GenBank reference samples, while the 4 divergent genotypes were not elaborated and marked as ND. Most of the Acanthamoeba genera were determined as likely dominating groups and clustered as T form within totally eight groups. Eight, seven and three subtypes were found as T4A, T15 and T11 genotypes, respectively while the remainings were ultimately found in four groups. Results confirming the predominance of T4A, which is known the most causative form, the presence in the pools. Despite being uncommon, N. fowleri, lovaniensis and australiensis were also observed among the surveyed pools. Conclusion The present study is descriptive and is not unique. However, this is the most comprehensive study of the molecular distribution sampling of thermophilic Acanthamoeba and Naegleria that confirmed and demonstrated their ubiquitous presence throughout Turkey. By this estimation, in some spas, the most and likely causative form Acanthamoeba including T4 and Naegleria fowleri has also been confirmed.
dc.description.sponsorshipCumhuriyet University, Sivas, Turkey [CUBAP T-614]
dc.description.sponsorshipIn doing this work, we have had substantial and kindly help from some friends. We are, first and especially, grateful to Dr. Mahir Korkmaz and Dr. Mahir Budak, who have provided some chemical and instrumentation support from their ongoing financial projects. We additionally thank M. Korkmaz for designing some primers. We are also grateful to MSc Student Bersan Secil Durel for the kind laboratory experiment support. This project was partially supported by CUBAP T-614, Cumhuriyet University, 58140 Sivas, Turkey.
dc.identifier.doi10.2478/s11686-019-00148-3
dc.identifier.endpage186
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.issue1
dc.identifier.pmid31797194
dc.identifier.scopus2-s2.0-85075948545
dc.identifier.scopusqualityQ3
dc.identifier.startpage174
dc.identifier.urihttps://doi.org/10.2478/s11686-019-00148-3
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30172
dc.identifier.volume65
dc.identifier.wosWOS:000500295300002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofActa Parasitologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcanthamoeba
dc.subjectNaegleria
dc.subjectPCR
dc.subjectGenotyping
dc.subject18SrRNA
dc.subjectPhylogenetic three
dc.titleGenotyping by Sequencing of Acanthamoeba and Naegleria Isolates from the Thermal Pool Distributed Throughout Turkey
dc.typeArticle

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