Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes

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dc.authoridCortes, Hernan/0000-0002-6147-4109
dc.authoridGonzalez Torres, Maykel/0000-0003-3899-4410
dc.authoridSharifi-Rad, Javad/0000-0002-7301-8151
dc.contributor.authorGutierrez-Ruiz, Stephany Celeste
dc.contributor.authorCortes, Hernan
dc.contributor.authorGonzalez-Torres, Maykel
dc.contributor.authorAlmarhoon, Zainab M.
dc.contributor.authorGurer, Eda Sonmez
dc.contributor.authorSharifi-Rad, Javad
dc.contributor.authorLeyva-Gomez, Gerardo
dc.date.accessioned2024-10-26T18:11:08Z
dc.date.available2024-10-26T18:11:08Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackgroundPolymeric nanoparticles can be used for wound closure and therapeutic compound delivery, among other biomedical applications. Although there are several nanoparticle obtention methods, it is crucial to know the adequate parameters to achieve better results. Therefore, the objective of this study was to optimize the parameters for the synthesis, purification, and freeze-drying of chitosan nanoparticles. We evaluated the conditions of agitation speed, anion addition time, solution pH, and chitosan and sodium tripolyphosphate concentration.ResultsChitosan nanoparticles presented an average particle size of 172.8 +/- 3.937 nm, PDI of 0.166 +/- 0.008, and zeta potential of 25.00 +/- 0.79 mV, at the concentration of 0.1% sodium tripolyphosphate and chitosan (pH 5.5), with a dripping time of 2 min at 500 rpm. The most representative factor during nanoparticle fabrication was the pH of the chitosan solution, generating significant changes in particle size and polydispersity index. The observed behavior is attributed to the possible excess of sodium tripolyphosphate during synthesis. We added the surfactants poloxamer 188 and polysorbate 80 to evaluate the stability improvement during purification (centrifugation or dialysis). These surfactants decreased coalescence between nanoparticles, especially during purification. The centrifugation increased the zeta potential to 40.8-56.2 mV values, while the dialyzed samples led to smaller particle sizes (152-184 nm). Finally, freeze-drying of the chitosan nanoparticles proceeded using two cryoprotectants, trehalose and sucrose. Both adequately protected the system during the process, and the sugar concentration depended on the purification process.ConclusionsIn Conclusion, we must consider each surfactant's benefits in formulations for selecting the most suitable. Also, it is necessary to do more studies with the molecule to load. At the same time, the use of sucrose and trehalose generates adequate protection against the freeze-drying process, even at a 5% w/v concentration. However, adjusting the percentage concentration by weight must be made to work with the CS-TPP NPs purified by dialysis.
dc.description.sponsorshipCONACYT [A1-S-15759]; PAPIIT-UNAM [IN204722]
dc.description.sponsorshipThis research was funded by CONACYT A1-S-15759 and PAPIIT-UNAM IN204722 to Gerardo Leyva-Gomez.
dc.identifier.doi10.1186/s13036-024-00403-w
dc.identifier.issn1754-1611
dc.identifier.issue1
dc.identifier.pmid38273413
dc.identifier.scopus2-s2.0-85182991842
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s13036-024-00403-w
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30535
dc.identifier.volume18
dc.identifier.wosWOS:001148750600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofJournal of Biological Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChitosan
dc.subjectFreeze-drying
dc.subjectNanoparticles
dc.subjectOptimization
dc.subjectPurification
dc.subjectTripolyphosphate
dc.titleOptimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes
dc.typeArticle

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