Thiamine Protects Glioblastoma Cells against Glutamate Toxicity by Suppressing Oxidative/Endoplasmic Reticulum Stress

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dc.authoridTaskiran, Ahmet Sevki/0000-0002-5810-8415
dc.contributor.authorErgul, Merve
dc.contributor.authorTaskiran, Ahmet Sevki
dc.date.accessioned2024-10-26T18:04:16Z
dc.date.available2024-10-26T18:04:16Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThiamine (vitamin B1), which is synthesized only in bacteria, fungi and plants and which humans should take with diet, participates in basic biochemical and physiological processes in a versatile way and its deficiency is associated with neurological problems accompanied by cognitive dysfunctions. The rat glioblastoma (C6) model was used, which was exposed to a limited environment and toxicity with glutamate. The cells were stressed by exposure to glutamate in the presence and absence of thiamine. The difference in cell proliferation was evaluated in the XTT assay. Oxidative stress (OS) markers malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels, as well as endoplasmic reticulum (ER) stress markers 78-kDa glucose-regulated protein (GRP78), activating transcription factor-4 (ATF-4), and C/EBP homologous protein (CHOP) levels, were measured with commercial kits. Apoptosis determined by flow cytometry was confirmed by 4',6-diamidino-2-phenylindole (DAPI) staining. At all concentrations, thiamine protects the cells and increased the viability against glutamate-induced toxicity. Thiamine also significantly decreased the levels of MDA, while increasing SOD and CAT levels. Moreover, thiamine reduced ER stress proteins' levels. Moreover, it lessened the apoptotic cell amount and enhanced the live-cell percentage in the flow cytometry and DAPI staining. As a result, thiamine may be beneficial nutritional support for individuals with a predisposition to neurodegenerative disorders due to its protective effect on glutamate cytotoxicity in glioblastoma cells by suppressing OS and ER stress.
dc.identifier.doi10.1248/cpb.c21-00169
dc.identifier.endpage839
dc.identifier.issn0009-2363
dc.identifier.issue9
dc.identifier.pmid34470947
dc.identifier.scopus2-s2.0-85114116467
dc.identifier.scopusqualityQ2
dc.identifier.startpage832
dc.identifier.urihttps://doi.org/10.1248/cpb.c21-00169
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28849
dc.identifier.volume69
dc.identifier.wosWOS:000692125000002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPharmaceutical Soc Japan
dc.relation.ispartofChemical & Pharmaceutical Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectthiamine
dc.subjectglutamate
dc.subjectoxidative stress
dc.subjectendoplasmic reticulum stress
dc.subjectC6 rat glioma
dc.titleThiamine Protects Glioblastoma Cells against Glutamate Toxicity by Suppressing Oxidative/Endoplasmic Reticulum Stress
dc.typeArticle

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