CRYOPRESERVATION AND ASSESSMENT OF HONAMLI AND HAIR BUCK SEMEN USING RESVERATROL

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dc.contributor.authorInanc, Muhammed Enes
dc.contributor.authorGungor, Sukru
dc.contributor.authorMart, Feyza Nur
dc.contributor.authorHerdogan, Mine
dc.contributor.authorCay, Hasan Ali
dc.contributor.authorKahraman, Durmus
dc.contributor.authorKorkmaz, Firat
dc.date.accessioned2025-05-04T16:45:35Z
dc.date.available2025-05-04T16:45:35Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBACKGROUND: Resveratrol (Res) (3,5,4 '-trihydroxystilbene) is a natural polyphenol that exhibits important biological activities. OBJECTIVE: To assess the effects of resveratrol (Res) on freeze-thawed survival of semen from Honamli and Hair Bucks. MATERIALS AND METHODS: Six bucks, aged 2-3 years (three from each breed), were included in the study. Semen was collected from each breed and mixed separately after removing seminal plasma. The mixed semen was diluted with different Res concentrations (0 mu M as control, 25 mu M, 50 mu M, 100 mu M, 500 mu M, and 1 mM) in Tris diluent and subjected to cryopreservation in liquid nitrogen vapor and frozen. After thawing, the samples were evaluated for motility and some spermatologic quality parameters by flow cytometry. RESULTS: Data were analyzed separately for Honamli and Hair breeds. The results showed that the Res 1 mM group had the lowest motility in all assessments (P<0.05). However, no significant differences were observed between the other Res and control groups (P>0.05). In terms of apoptosis, Hair bucks exhibited a statistically significant difference in late apoptotic parameters, with the control showing the highest values (P<0.05). The Res 25 mu M group (similar to the control group) showed lower mitochondrial oxidative stress than the Res 1 mM group (P<0.05). CONCLUSION: Res at a dose of 1 mM did not protect most sperm functional and biochemical parameters except for apoptosis and performed worse than the control group. When all parameters are evaluated collectively, concentrations lower than 1 mM should be used for freezing Honamli and Hair Buck semen with resveratrol.
dc.description.sponsorshipBurdur Mehmet Akif Ersoy University Scientific Research Projects Coordination [0726-MP-21/2017K12-41003]
dc.description.sponsorshipThis research was supported by Burdur Mehmet Akif Ersoy University Scientific Research Projects Coordination, Project No. 0726-MP-21/2017K12-41003.
dc.identifier.doi10.54680/fr25110110312
dc.identifier.endpage21
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue1
dc.identifier.pmid40089812
dc.identifier.scopus2-s2.0-85213880451
dc.identifier.scopusqualityQ3
dc.identifier.startpage14
dc.identifier.urihttps://doi.org/10.54680/fr25110110312
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35130
dc.identifier.volume46
dc.identifier.wosWOS:001390176400002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryDiğer
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectbuck
dc.subjectcryopreservation
dc.subjectresveratrol
dc.subjectsemen
dc.subjectspermatological parameters
dc.titleCRYOPRESERVATION AND ASSESSMENT OF HONAMLI AND HAIR BUCK SEMEN USING RESVERATROL
dc.typeOther

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