Molecular mechanism of anticancer effect of heat shock protein 90 inhibitor BIIB021 in human bladder cancer cell line

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dc.contributor.authorAsdemir, Aydemir
dc.contributor.authorOzgur, Aykut
dc.date.accessioned2024-10-26T18:07:25Z
dc.date.available2024-10-26T18:07:25Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBladder cancer is a type of urologic malignancy that exhibits significant morbidity, mortality, and treatment costs. Inhibition of heat shock protein 90 (HSP90) activity has been a promising pharmacological strategy for blocking of bladder cancer pathogenesis. BIIB021 is a next-generation HSP90 inhibitor which interrupts ATP hydrolysis process of HSP90 and inhibits the stabilization and correct folding of client proteins. In current study, we aimed to investigate the molecular mechanism of the anticancer activity of BIIB021 in human bladder cancer T24 cells. Our results revealed that nanomolar concentration of BIIB021 decreased viability of T24 cell. BIIB021 downregulated HSP90 expression in T24 cells and inhibited the refolding activity of luciferase in the presence of T24 cell lysate. PCR array data indicated a significant alteration in transcript levels of cancer-related genes involved in metastases, apoptotic cell death, cell cycle, cellular senescence, DNA damage and repair mechanisms, epithelial-to-mesenchymal transition, hypoxia, telomeres and telomerase, and cancer metabolism pathways in T24 cells. All findings hypothesize that BIIB021 could exhibit as effective HSP90 inhibitor in the future for treatment of bladder cancer patients.
dc.description.sponsorshipSivas Cumhuriyet University
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s00210-024-02950-x
dc.identifier.endpage5177
dc.identifier.issn0028-1298
dc.identifier.issn1432-1912
dc.identifier.issue7
dc.identifier.pmid38240781
dc.identifier.scopus2-s2.0-85182677495
dc.identifier.scopusqualityQ2
dc.identifier.startpage5167
dc.identifier.urihttps://doi.org/10.1007/s00210-024-02950-x
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29477
dc.identifier.volume397
dc.identifier.wosWOS:001145766900005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofNaunyn-Schmiedebergs Archives of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBladder cancer
dc.subjectChemotherapy
dc.subjectHSP90
dc.subjectChaperone
dc.subjectT24
dc.subjectBIIB021
dc.titleMolecular mechanism of anticancer effect of heat shock protein 90 inhibitor BIIB021 in human bladder cancer cell line
dc.typeArticle

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