Bromelain Protects Against PTZ-Induced Glial Damage and Inflammation: An In Vitro and In Silico Study

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dc.authoridJoha, Ziad/0000-0001-8520-3760
dc.contributor.authorJoha, Ziad
dc.contributor.authorBasgoz, Neslihan
dc.contributor.authorOzgur, Aykut
dc.contributor.authorTaskiran, Ahmet Sevki
dc.date.accessioned2025-05-04T16:47:23Z
dc.date.available2025-05-04T16:47:23Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study aimed to investigate how bromelain protects glial cells from pentylenetetrazole (PTZ)-induced damage, focusing on its anti-inflammatory effects. C6 glioma cells were treated with PTZ, bromelain, or a combination of PTZ and bromelain. The interactions of bromelain with iNOS (Inducible Nitric Oxide Synthase) and COX2 (Cyclooxygenase-2) were investigated using molecular docking calculations. Cell viability was measured using the XTT (Methoxynitrosulfophenyl-Tetrazolium Carboxanilide) assay. iNOS, NO (Nitric Oxide), and COX2 levels were assessed using ELISA and immunofluorescence staining. Bromelain at 50 and 100 mu g/mL significantly increased cell viability (p < 0.001). On the other hand, bromelain at 50 g/mL reduced inflammation, as indicated by lower levels of NO, iNOS, and COX2 (p < 0.001). In-silico predictions suggest that bromelain can effectively target iNOS and COX2, key inflammatory proteins. These findings indicate that bromelain protects glial cells by exerting anti-inflammatory effects. However, further research is needed to understand the underlying mechanisms fully.
dc.identifier.doi10.1007/s12013-025-01703-8
dc.identifier.issn1085-9195
dc.identifier.issn1559-0283
dc.identifier.pmid40000586
dc.identifier.scopus2-s2.0-85218904645
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s12013-025-01703-8
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35588
dc.identifier.wosWOS:001431254200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc
dc.relation.ispartofCell Biochemistry and Biophysics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectBromelain
dc.subjectPTZ
dc.subjectInflammation
dc.subjectNeuroprotection
dc.subjectGlial Cells
dc.titleBromelain Protects Against PTZ-Induced Glial Damage and Inflammation: An In Vitro and In Silico Study
dc.typeArticle

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