Investigation of the mechanisms involved in anticancer effect of glucosamine sulfate on SH-SY5Y cell line

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dc.authorid0000-0002-4419-1385tr
dc.contributor.authorŞahin Bilal
dc.contributor.authorGüneş Handan
dc.contributor.authorÖztürk Ayşegül
dc.date.accessioned2023-06-23T09:42:46Z
dc.date.available2023-06-23T09:42:46Z
dc.date.issued2022tr
dc.departmentTıp Fakültesitr
dc.description.abstractAim: Glucosamine derivatives have been found to have anticancer effects in many cancer cell lines in previous investigations. The effect of glucosamine sulfate on neuroblastoma, however, is uncertain. The potential cytotoxic effects of glucosamine sulfate on the SH-SY5Y cell line were investigated in this study. The underlying mechanisms of this cytotoxicity have also been studied. Material and methods: In this study, the SH-SY5Y cell lines were used. The cells were treated with various concentrations of glucosamine sulfate (0.3125, 0.625, 1.25 and 2.5 μg/mL) and the viability of the cells was determined using the XTT assay after 24 hours. The quantities of cleaved PARP, BCL-2, 8-Hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant in the cells were determined by ELISA kits. Results: At doses of 0.3125, 0.625, 1.25 and 2.5 μg/mL, glucosamine sulfate dramatically reduced cell viability in SH-SY5Y cells (p<0.001). ELISA tests demonstrated that 1.25 μg/mL glucosamine sulfate considerably increased the amounts of 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP and total oxidant. However, 1.25 μg/mL glucosamine sulfate treatment did not change the quantity of BCL-2 protein. Conclusions: Altogether, glucosamine sulfate produced considerable cytotoxicity in SH-SY5Y cells by triggering oxidative stress, inducing DNA damage, and finally causing apoptosis. In addition, more research is needed to determine the efficacy of glucosamine sulfate as an anticancer drug in the treatment of neuroblastomatr
dc.identifier.citationSahin, B., Gunes, H., & Ozturk, A. (2022). Investigation of the mechanisms involved in anticancer effect of glucosamine sulfate on SH-SY5Y cell line. Bratislavske lekarske listy, 123(5), 366–371. https://doi.org/10.4149/BLL_2022_058tr
dc.identifier.doi10.4149/BLL_2022_058en_US
dc.identifier.endpage371tr
dc.identifier.issue5tr
dc.identifier.pmid35420883en_US
dc.identifier.scopus2-s2.0-85128253281en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage366tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14078
dc.identifier.volume123tr
dc.identifier.wosWOS:000819938300010en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBratislavske lekarske listytr
dc.relation.publicationcategoryUlusal Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/openAccesstr
dc.subjectSH-SY5Y cell; apoptosis ELISA.; cell viability; dna damage; glucosamine sulfate; oxidative stress.tr
dc.titleInvestigation of the mechanisms involved in anticancer effect of glucosamine sulfate on SH-SY5Y cell lineen_US
dc.typeArticleen_US

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