The Evaluation of Anticancer, Antioxidant, Antidiabetic and Anticholinergic Potentials of Endemic Rhabdosciadium microcalycinum Supported by Molecular Docking Study

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dc.authoridTaslimi, Parham/0000-0002-3171-0633
dc.authoridErdogan, Mehmet Kadir/0000-0002-1579-5737
dc.contributor.authorErdogan, Mehmet Kadir
dc.contributor.authorGundogdu, Ramazan
dc.contributor.authorYapar, Yakup
dc.contributor.authorGecibesler, Ibrahim Halil
dc.contributor.authorKirici, Mahinur
dc.contributor.authorBehcet, Lutfi
dc.contributor.authorTuzun, Burak
dc.date.accessioned2024-10-26T18:08:59Z
dc.date.available2024-10-26T18:08:59Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractLung cancer is the most common cancer worldwide and the leading cause of cancer-related death. Plant-derived natural products and compounds are an inspiring source of chemotherapeutic agents. Alzheimer's disease (AD) is linked to the decline of acetylcholine (ACh) effects in the brain, so acetylcholinesterase (AChE) inhibitors are important in the treatment of AD. In this study, the chemical components and bioactivity of ethanolic extract of Rhabdosciadium microcalycinum (RM) was investigated by various methods. The lipophilic components of RM was determined by gas chromagraphy-mass spectrometry (GC-MS). Antioxidant activicty tests were evaluated with FCAP (Ferrous chelating antioxidant power assay), FRAP (Ferric reducing antioxidant power assay), ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay) and DPPH (2,2-diphenyl-1-picrylhydrazyl assay). The anticancer effect of RM was evaluated by WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate), colony formation, wound healing and CDDE (cell death detection Elisa) analysis on A549 lung cancer cells. Enzyme inhibition effects of RM was determined against AChE and alpha-glycosidase (AG) enzymes. According to the results of molecular docking found in the study; With a docking score of -4.56 for HP5 protein, -6.23 for BXO protein, -5.25 for AFI protein, -3.90 for gly protein and -7.74 for AChE protein, Telecinobufagin molecule was found to have higher activity than other molecules. After docking calculations, Absorption, Distribution, Metabolism, Excretion and Toxicity (ADME/T) analysis was performed to examine the effects and reactions of molecules on human metabolism. RM inhibited the viability of A549 cells dose-dependent manner (IC50=117.15 +/- 8.58 mu g/mL), and it was also observed that RM suppressed colony formation, prevented cell migration and directed the cells to apoptosis. RM has important lipophilic components, and significant antioxidant and enzyme inhibition potential. IC50 values of RM for AChE and AG enzymes were found as 35.86 mg/mL, and 10.14 mg/mL, respectively. In conclusion, the findings of this study reveal that ethanol extract from the aerial part of Rhabdosciadium microcalycinum may be a potential therapeutic agent for the treatment of human lung cancer and Alzheimer's disease, due to its phytochemical components.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Bingol University [BAP-FEF.2021.008]; Scientific Research Project Fund of Sivas Cumhuriyet University [RGD-020]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Bingol University (Project no: BAP-FEF.2021.008), and the Scientific Research Project Fund of Sivas Cumhuriyet University (Project no: RGD-020). This research was made possible by TUBITAK ULAKBIM, High Performance and Grid Computing Center (TR-Grid e-Infrastructure).
dc.identifier.doi10.1002/slct.202200400
dc.identifier.issn2365-6549
dc.identifier.issue17
dc.identifier.scopus2-s2.0-85130034508
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/slct.202200400
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29870
dc.identifier.volume7
dc.identifier.wosWOS:000789869900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistryselect
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectantioxidant
dc.subjectCancer
dc.subjectenzymes
dc.subjectinhibition
dc.subjectmolecular docking
dc.subjectRhabdosciadium microcalycinum
dc.titleThe Evaluation of Anticancer, Antioxidant, Antidiabetic and Anticholinergic Potentials of Endemic Rhabdosciadium microcalycinum Supported by Molecular Docking Study
dc.typeArticle

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