In Vitro Neuroprotective Effect Evaluation of Donepezil-Loaded PLGA Nanoparticles-Embedded PVA/PEG Nanofibers on SH-SY5Y Cells and AP-APP Plasmid Related Alzheimer Cell Line Model

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dc.contributor.authorGuler, Ece
dc.contributor.authorYekeler, Humeyra Betul
dc.contributor.authorUner, Burcu
dc.contributor.authorDogan, Murat
dc.contributor.authorAsghar, Asima
dc.contributor.authorIkram, Fakhera
dc.contributor.authorYazir, Yusufhan
dc.date.accessioned2025-05-04T16:47:31Z
dc.date.available2025-05-04T16:47:31Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractRecently developed nanoparticles and nanofibers present new brain-specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)-loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for sublingual administration. SEM images showed produced drug-loaded and pure nanofibers, which have sizes between 978 and 1123 nm, demonstrated beadless morphology and homogeneous distribution. FT-IR, XRD, and DSC results proved the produced nanoparticles and fibers to consist of the DO and other polymers. The in vitro drug release test presented that the release profile of DO is completed at the end of the 18th day. It is released by the first order kinetic model. DNPF has an ultra-fast release profile via its disintegration within 2 sec, which proved itself to be suitable for the administration sublingually. All samples presented above approximate to 90% cell viability via their non-toxic natures on SH-SY5Y human neuroblastoma cells by using Alamar blue assay. The anti-Alzheimer effects of DO, DNP, and DNPF are evaluated on the A beta(1-42)-induced SH-SY5Y cells at 1, 5, and 10 mu M as treatment groups. The 1 mu M dosage exhibited the most significant neuroprotective effects, which showed enhanced cellular uptake and superior modulation of Alzheimer's-related proteins, including tau and A beta.
dc.identifier.doi10.1002/mame.202400160
dc.identifier.issn1438-7492
dc.identifier.issn1439-2054
dc.identifier.issue3
dc.identifier.scopus2-s2.0-86000434928
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mame.202400160
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35658
dc.identifier.volume310
dc.identifier.wosWOS:001340937200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofMacromolecular Materials and Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250504
dc.subjectAlzheimer's disease
dc.subjectAlzheimer's disease cell model
dc.subjectAP-APP plasmid transfection
dc.subjectdonepezil
dc.subjectdrug delivery
dc.subjectnanofiber
dc.subjectnanoparticle
dc.titleIn Vitro Neuroprotective Effect Evaluation of Donepezil-Loaded PLGA Nanoparticles-Embedded PVA/PEG Nanofibers on SH-SY5Y Cells and AP-APP Plasmid Related Alzheimer Cell Line Model
dc.typeArticle

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