Purification and characterisation of 1-dodecanol from an isolate of Streptomyces viridodiastaticus

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dc.authoridAksu, Aysun/0000-0001-9168-2745
dc.contributor.authorCetinkaya, Serap
dc.contributor.authorYenidunya, Ali Fazil
dc.contributor.authorAksu, Aysun
dc.contributor.authorCelik, Muhammed Safa
dc.date.accessioned2024-10-26T18:02:31Z
dc.date.available2024-10-26T18:02:31Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractOne-dodecanol was identified as the predominant secondary metabolite produced by a novel isolate of Streptomyces viridodiastaticus. A total secondary metabolite extract was electrophoresed, along with a prestained protein ladder (from 10 to 180 kDa), in a 10% SDS-polyacrylamide gel. Two prominent bands obtained were aligned with 25 and 10 kDa bands of the protein marker. These two secondary metabolite bands were also visualized by thin layer chromatography. The two bands, lower band (C1) and upper band (C2), in the polyacrylamide gel were sliced off and their metabolite content was eluted in n-butanol. Elution samples were analysed by GC-MS. Results indicated that the lower C1 band mostly contained 1-dodecanol, approximately 93%. The lower band also contained a 1-dodecanol-like metabolite, approximately 55%. The elution samples were also analysed by FTIR and the results were in accordance with those of the GC-MS.
dc.identifier.doi10.1016/j.bcab.2021.102013
dc.identifier.issn1878-8181
dc.identifier.scopus2-s2.0-85105322315
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.bcab.2021.102013
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28178
dc.identifier.volume34
dc.identifier.wosWOS:000663764300006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiocatalysis and Agricultural Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject1-Dodecanol
dc.subjectCharacterisation
dc.subjectElectrophoresis
dc.subjectPurification
dc.subjectSDS-PAGE
dc.subjectStreptomyces viridodiastaticus
dc.titlePurification and characterisation of 1-dodecanol from an isolate of Streptomyces viridodiastaticus
dc.typeArticle

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