An FPSE-HPLC-PDA method for rapid determination of solar UV filters in human whole blood, plasma and urine

Basit Öğe Kaydı
dc.authoridKabir, Abuzar -- 0000-0002-3740-8857; Locatelli, Marcello -- 0000-0002-0840-825Xen_US
dc.contributor.authorLocatelli, Marcello
dc.contributor.authorFurton, Kenneth G.
dc.contributor.authorTartaglia, Angela
dc.contributor.authorSperandio, Elena
dc.contributor.authorUlusoy, Halal I.
dc.contributor.authorKabir, Abuzar
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T09:37:01Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T09:37:01Z
dc.date.issued2019
dc.department[Locatelli, Marcello -- Tartaglia, Angela -- Sperandio, Elena] Univ Chieti Pescara G dAnnunzio, Dept Pharm, Chieti, Italy -- [Furton, Kenneth G. -- Kabir, Abuzar] Florida Int Univ, Dept Chem & Biochem, Int Forens Res Inst, Miami, FL 33199 USA -- [Ulusoy, Halal I.] Cumhuriyet Univ, Dept Analyt Chem, Fac Pharm, Sivas, Turkeyen_US
dc.description.abstractThis paper reports a novel fabric phase sorptive extraction-high performance liquid chromatography-photodiode array detection (FPSE-HPLC-PDA) method for the simultaneous extraction and analysis of six benzophenone derivative UV filters including benzophenone (BZ); 5-benzoyl-4-hydroxy-methoxybenzenesulfonic acid (BP-4); bis(4-hydroxyphenyl)methanone (4-DHB); bis(2,4-dihydroxyphenyl)methanone (BP-2); (2,4-dihydroxybenzophenone) (BP-1) and 2,2'-dihydroxy-4-methoxybenzophenone (DHMB) in human whole blood, plasma and urine samples. Chromatographic separation method was conducted using a Spherisorb ODS 1 (C-18) column in isocratic elution mode with a run time < 25 min. The FPSE-HPLC-PDA method was validated in the range from 0.1 to 10 mu g/mL for all the UV filter compounds. Propyl 4-hydroxybenzoate (also known as propyl paraben) was used as the internal standard (IS). The limit of quantification was found to be 0.1 mu g/mL and the weighted-matrix matched standard calibration curves of six UV filters showed a good linearity up to a concentration of 10 mu g/mL. This new approach exhibits high potential for direct adaptation as a rapid, robust and green analytical tool for several applications, e.g. in the current sample preparation practices used in many bioanalytical fields including pharmacokinetics (PK), pharmacodynamics (PD), therapeutic drug monitoring (TDM), clinical and forensic toxicology, disease diagnosis and drug discovery. Additionally, in the present work was highlighted that applying innovative extraction and clean up procedures before instrumental analysis by means of a well-known, rugged, cheap, and diffused configurations (e.g. HPLC-PDA), could be possible to validate methods that shows analytical performances comparable to more expensive and complex instrumentations (e.g. LC-MS/MS) that require trained personnel, high maintenance costs and a deep knowledge of analytical problems that could be encountered.en_US
dc.description.sponsorshipMIUR, University of Chieti - Pescara "G. d'Annunzio", Chieti, Italyen_US
dc.description.sponsorshipThis work was supported by grant MIUR ex 60%, University of Chieti - Pescara "G. d'Annunzio", Chieti, Italy.en_US
dc.identifier.doi10.1016/j.jchromb.2019.04.028en_US
dc.identifier.endpage50en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid31005773en_US
dc.identifier.scopus2-s2.0-85064325796en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage40en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jchromb.2019.04.028
dc.identifier.urihttps://hdl.handle.net/20.500.12418/5873
dc.identifier.volume1118en_US
dc.identifier.wosWOS:000469153600006en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFPSE-HPLC-PDAen_US
dc.subjectMethod validationen_US
dc.subjectBenzophenone-derivative UV filtersen_US
dc.subjectWhole blooden_US
dc.subjectPlasma and urineen_US
dc.subjectHuman exposureen_US
dc.subjectClinical and diagnostic applicationsen_US
dc.titleAn FPSE-HPLC-PDA method for rapid determination of solar UV filters in human whole blood, plasma and urineen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksyonu
Scopus İndeksli Yayınlar Koleksiyonu