Impact of Trolox Supplementation on the Cryopreservation of Honamli Buck Semen

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dc.contributor.authorGungor, Sukru
dc.contributor.authorInanc, Muhammed Enes
dc.contributor.authorUslu, Baris Atalay
dc.contributor.authorBurca, Ahmet Burak
dc.contributor.authorAta, Ayhan
dc.date.accessioned2025-05-04T16:46:53Z
dc.date.available2025-05-04T16:46:53Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractCryopreservation of buck semen is essential in animal breeding but often damages sperm viability and integrity. The Honamli breed, a hardy Turkish goat, can benefit from improved freezing techniques using antioxidants such as Trolox (T). This study explores the effects of varying T concentrations on Honamli buck semen, assessing parameters such as motility, viability, and membrane integrity to enhance post-thaw quality. Findings support T's potential to improve semen extender formulations for preserving Honamli genetics.This study aims to freeze Honamli buck semen with T and to evaluate in vitro spermatological parameters. Three Honamli bucks, aged 2-3 years, were used in the study. Semen was collected from the bucks and mixed after removing seminal plasma. The mixed semen was diluted with a tris egg yolk extender containing three different concentrations of T (0.25 mM, 0.5 mM, and 1 mM) and control (0 mM). The diluted semen was equilibrated for 2 hours at +4 degrees and subjected to cryopreservation in liquid nitrogen vapor (-120 degrees C for 12 minutes) and frozen. After thawing (37 degrees C water bath for 30 seconds), the groups were evaluated at flow cytometric analysis for viability (SYBR/propidium iodide [PI]), plasma membrane acrosome integrity (FITC-PNA/PI), and mitochondrial membrane potential (JC-1), plasma membrane integrity (hypo-osmotic swelling test), microscopic evaluations for motility and morphological integrity (abnormal spermatozoa rate). The 0.5 T and 0.25 T groups showed significant improvements in motility compared with the control group (p < 0.05). The control group had the lowest plasma membrane integrity (p < 0.05). The highest morphological integrity was observed in the T groups compared with the control group (p < 0.05). In conclusion, supplementing T in buck semen extenders benefits spermatological parameters; particularly, 0.25 and 0.5 mM T could be used in Tris semen extenders during the cryopreservation process.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK/2209 A) [1919B011802783/2018]
dc.description.sponsorshipThis study was financed under the project supported by the Scientific and Technological Research Council of Turkey (TUBITAK/2209 A; Project No. 1919B011802783/2018).
dc.identifier.doi10.1089/bio.2024.0161
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.pmid40042107
dc.identifier.scopus2-s2.0-86000184258
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1089/bio.2024.0161
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35390
dc.identifier.wosWOS:001438099800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofBiopreservation and Biobanking
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectantioxidant
dc.subjectHonamli
dc.subjectbuck semen
dc.subjectTrolox
dc.titleImpact of Trolox Supplementation on the Cryopreservation of Honamli Buck Semen
dc.typeArticle

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