Preparation and characterization of lysozyme loaded cryogel for heavy metal removal

Basit Öğe Kaydı
dc.authorid0000-0001-9139-1884tr
dc.contributor.authorAkkaya, Birnur
dc.date.accessioned2024-02-27T06:27:50Z
dc.date.available2024-02-27T06:27:50Z
dc.date.issued2023tr
dc.departmentFen Fakültesitr
dc.description.abstractIn the present study, monolithic poly(N-isopropylacrylamide-acrylamide)-acrilic acid (poly(npam-aam)-aac) cryogels were made. Swelling tests, SEM, XRD, and ATR-FTIR analyses revealed distinct cryogel and lysozyme-loaded cryogel properties. The equilibrium swelling degree was 6.2 g H2O/g cryogel. The created poly(npam-aam)-aac with pores of 10–100 μm was obviously seen in SEM images. Lysozyme adsorption capacity on poly(npam-aam)-aac was found to be 260 mg/g at pH 7.4 and 40 °C. After that, we used lysozyme adsorbed cryogel for the removal of the model heavy metal ion (cadmium). A series of pH, duration, and ionic strengths were used to conduct Cd2+ adsorption experiments. The results showed that the new adsorbent had a considerable chemical affinity for Cd2+ ions in its ability to bind them under eye ocular conditions (pH 7.4, 32–36 °C, 0,15 M NaCl). The traditional Langmuir adsorption model was the most suitable, achieving maximum uptake of ∼185 mg/g. Chemical adsorption was found to be the rate-controlling step, and the process was also compatible with the pseudo-second-order model. For the treatment of ocular pathologies, the most effective enzyme, lysozyme, must show its function. That is why there is a need for using lysozyme, and lysozyme is selected as a lignad to adsorb heavy metal ions because of its high heavy metal binding affinity. This material could be used for the treatment of ocular pathologies in the future.tr
dc.description.sponsorship...tr
dc.identifier.citation-tr
dc.identifier.doi10.1016/j.ijbiomac.2023.127494en_US
dc.identifier.endpage494tr
dc.identifier.issue8tr
dc.identifier.pmid37858643en_US
dc.identifier.scopus2-s2.0-85175088549en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage127tr
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S014181302304391X?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14339
dc.identifier.volume253tr
dc.identifier.wosWOS:001105232700001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInternational Journal of Biological Macromoleculestr
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/restrictedAccesstr
dc.subjectCryogel, Eye diseases, Heavy metal, Immobilization, Lysozymetr
dc.titlePreparation and characterization of lysozyme loaded cryogel for heavy metal removalen_US
dc.typeArticleen_US

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