Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected

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dc.authoridKARATAS, Ozhan/0000-0002-2778-8059
dc.contributor.authorKaratas, Ozhan
dc.contributor.authorOzkaraca, Mustafa
dc.contributor.authorAtasoy, Mustafa O.
dc.date.accessioned2025-05-04T16:47:29Z
dc.date.available2025-05-04T16:47:29Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study aimed to detect the presence of bovine papillomavirus (BPV) in the testicular tissue of bulls over 1-year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, gamma H2AX and cytochrome c expressions were evaluated by immunohistochemical and immunofluorescent methods in samples positive for BPV agent. In this study, 100 testicular specimens that did not show any macroscopic papilloma findings were collected. Testicular tissues were collected and examined using histologically and molecularly methods. In the present study, immunopositivity was detected in 6 (6%) samples with the IHC method and in 11 (11%) samples with the IF method. In addition, BPV positivity was detected in four (4%) samples by the PCR method and four (4%) samples determined to be BPV positive by PCR also showed immunopositivity in IHC and IF methods. gamma H2AX immunopositivity was seen in two of the BPV-positive samples by the IHC method, and gamma H2AX immunopositivity was observed in four of the samples determined to be positive for the BPV agent by the IF method. Cytochrome c immunopositivity was found in all the samples determined positive for the BPV agent. When the findings were evaluated, the presence of the BPV agent in the testicular tissues of cattle that did not have macroscopic papillomas was revealed for the first time. It was thought that the BPV agent caused double-stranded DNA breaks and increased cytochrome c expression.
dc.description.sponsorshipTUBITAK
dc.description.sponsorshipThis study was supported by Sivas Cumhuriyet University Scientific Research Projects (CUBAP) under the code V-106. This study was produced from the doctoral thesis.
dc.identifier.doi10.1002/vms3.70132
dc.identifier.issn2053-1095
dc.identifier.issue1
dc.identifier.pmid39792070
dc.identifier.scopus2-s2.0-85214517602
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/vms3.70132
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35643
dc.identifier.volume11
dc.identifier.wosWOS:001393809200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofVeterinary Medicine and Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250504
dc.subjectcytochrome c
dc.subjectimmunofluorescence
dc.subjectimmunohistochemistry
dc.subjectPCR
dc.subjecttesticular tissue
dc.subjectgamma H2AX
dc.titleInvestigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected
dc.typeArticle

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