Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics

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dc.contributor.authorDogan, Murat
dc.date.accessioned2024-10-26T18:00:31Z
dc.date.available2024-10-26T18:00:31Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjective: In this study, it was aimed to make in vitro characterization of the formulations by preparing hydrogel formulations using chitosan, a biocompatible and natural polymer, and PVP, a synthetic polymer. In addition, the effects of hydrogels containing FGF on the proliferation of keratinocyte cells were investigated. Methods: Within the scope of the study, hydrogels with different properties were prepared and their water absorption capacity, and viscosities were examined. In addition, the hardness, adhesiveness, cohesiveness, and elasticity properties of hydrogels were investigated. The 3 - (4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) test was applied to evaluate the toxicity of hydrogels on keratinocyte cell lines. Results: It was observed that hydrogel formulations have high water absorption capacity and suitable viscosity values. In addition, the mechanical characterization results showed that the hydrogels have suitable mechanical properties. According to the results of in vitro cell culture studies, it has been observed that hydrogels stimulate the proliferation of keratinocyte cells. Conclusion: Results showed that the mechanical properties of hydrogels containing FGF are suitable for application and according to the results of in vitro cell culture studies, hydrogels can be used in wound healing studies because they increase keratinocyte cell proliferation.
dc.identifier.doi10.33808/clinexphealthsci.972758
dc.identifier.endpage422
dc.identifier.issn2459-1459
dc.identifier.issue2
dc.identifier.startpage418
dc.identifier.trdizinid532920
dc.identifier.urihttps://doi.org/10.33808/clinexphealthsci.972758
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/532920
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27729
dc.identifier.volume12
dc.identifier.wosWOS:000823273900020
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherMarmara Univ, Inst Health Sciences
dc.relation.ispartofClinical and Experimental Health Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChitosan
dc.subjectIn vitro characterization
dc.subjectHydrogel
dc.subjectCell proliferation
dc.titlePreparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics
dc.typeArticle

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