A different approach to diagnosis of subclinical mastitis: milk arginase activity

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dc.authoridKANDEMIR, Fatih Mehmet/0000-0002-8490-2479
dc.contributor.authorKandemir, Fatih M.
dc.contributor.authorYuksel, Murat
dc.contributor.authorOzdemir, Necmi
dc.contributor.authorDeveci, Huseyin
dc.date.accessioned2024-10-26T18:09:07Z
dc.date.available2024-10-26T18:09:07Z
dc.date.issued2013
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThe aim of this study was to determine the relationships between subclinical mastitis and milk arginase activity in dairy cows. Thirty, various breed 4-8 year old cows from Firat University Animal Hospital were used in the study. Subclinical mastitis was diagnosed by the California Mastitis Test (CMT) combined with bacteriological examination of milk samples. The subclinical mastitis group consisted of fifteen clinically healthy but CMT and microbiologically positive animals. CMT and microbiologically negative animals (n = 15) served as the control group. Arginase activity in milk samples was measured by spectrophotometer using the thiocemicarbazide diacetylmonoxime urea (TDMU) method and protein was determined with the folin phenol reagent. Arginase activity in the milk of animals with subclinical mastitis (0.26 +/- 0.07 U/mg protein) significantly increased compared to the arginase activity in the milk from healthy animals in the control group (0.10 +/- 0.03 U/mg protein). Milk enzyme activity in the mildly severe disease (0.26 +/- 0.01 U/mg protein) was significantly lower than in the moderately severe disease (0.30 +/- 0.01 U/mg). No relationship was found between the nature of the bacterial infection of the cows with subclinical mastitis and their milk arginase activity. It was concluded that assay of milk arginase activity may be used as an additional laboratory method for the diagnosis of subclinical mastitis.
dc.identifier.endpage610
dc.identifier.issn0372-5480
dc.identifier.issn1331-8055
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84890108062
dc.identifier.scopusqualityQ3
dc.identifier.startpage603
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29960
dc.identifier.volume83
dc.identifier.wosWOS:000330252800003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUniv Zagreb Vet Faculty
dc.relation.ispartofVeterinarski Arhiv
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectsubclinical mastitis
dc.subjectarginase
dc.subjectcow
dc.subjectCalifornia mastitis test
dc.titleA different approach to diagnosis of subclinical mastitis: milk arginase activity
dc.typeArticle

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