Effect of ovarian stimulation on the expression of piRNA pathway proteins

Basit Öğe Kaydı
dc.authoridSari, Ismail/0000-0003-3732-2102
dc.authoridTaskiran, Ahmet Sevki/0000-0002-5810-8415
dc.contributor.authorSari, Ismail
dc.contributor.authorGumus, Erkan
dc.contributor.authorTaskiran, Ahmet Sevki
dc.contributor.authorSokmensuer, Lale Karakoc
dc.date.accessioned2024-10-26T18:11:03Z
dc.date.available2024-10-26T18:11:03Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractPIWI-interacting RNAs (piRNAs) play an important role in gametogenesis, fertility and embryonic development. The current study investigated the effect of different doses of pregnant mare serum gonadotrophin/human chorionic gonadotrophin (PMSG/hCG) and repeated ovarian stimulation (OS) on the expression of the Mili, Miwi, Mael, Tdrd1, Tdrd9, qnd Mitopld genes, which have crucial roles in the biogenesis and function of piRNAs. Here, we found that after treatment with 7.5 I.U. PMSG/hCG and two repeated rounds of OS, both the mRNA and protein levels of Tdrd9, Tdrd1 and Mael showed the greatest decrease in the ovarian tissue, but the plasma E-2 levels showed the strongest increases (p< 0.05). However, we found that the Mitopld, Miwi and Mili gene levels were decreased significantly after treatment with 12.5 I.U. PMSG/hCG. Our results suggested that exogenous gonadotropin administration leads to a significant decrease in the expression of the Mili, Miwi, Mael, Tdrd1, Tdrd9 and Mitopld genes, which are critically important in the piRNA pathway, and the changes in the expression levels of Tdrd9, Tdrd1 and Mael may be associated with plasma E-2 levels. New comprehensive studies are needed to reduce the potential effects of OS on the piRNA pathway, which silences transposable elements and maintains genome integrity, and to contribute to the safety of OS.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [116s674]
dc.description.sponsorshipThis work was supported by TUBITAK (The Scientific and Technological Research Council of Turkey) under grand number 116s674.
dc.identifier.doi10.1371/journal.pone.0232629
dc.identifier.issn1932-6203
dc.identifier.issue5
dc.identifier.pmid32365144
dc.identifier.scopus2-s2.0-85084277001
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0232629
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30471
dc.identifier.volume15
dc.identifier.wosWOS:000536959700048
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleEffect of ovarian stimulation on the expression of piRNA pathway proteins
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksyonu
Scopus İndeksli Yayınlar Koleksiyonu