Assessment of vaginal and endometrial microbiota by real?time PCR in women with unexplained infertility

Basit Öğe Kaydı
dc.authorid0000-0003-4725-043Xtr
dc.contributor.authorSezer, Özlem
dc.contributor.authorCaliskan, Canan
dc.contributor.authorÇelik, Samettin
dc.contributor.authorKılıç, Süleyman Sırrı
dc.contributor.authorKuruoglu, Tuba
dc.contributor.authorUnluguzel Ustun, Goksenin
dc.date.accessioned2023-06-23T05:34:50Z
dc.date.available2023-06-23T05:34:50Z
dc.date.issued2022/1tr
dc.departmentTıp Fakültesitr
dc.description.abstractAbstract Aim Microbiota of the reproductive tract may be associated with unexplained infertility in women. We aimed to determine the derangements of vaginal and endometrial microbiota related to unexplained infertility by real-time polymerase chain reaction (real-time PCR) microbiota analyses of vaginal and endometrial samples of the unexplained infertility patients and fertile women. Materials and Methods Twenty-six women with unexplained infertility and 26 age-matched fertile women were included. Vaginal and endometrial samples were obtained in the mid-menstrual cycle for analysis by quantitative real-time PCR method. Results The proportion of lactobacilli-impaired microbiota was significantly higher in the vaginal samples of unexplained infertility patients (76.9% vs. 26.9%; p < 0.001). Those with impaired lactobacilli microbiota of vaginal samples had an increased risk of 9.048 times for infertility than those with normal lactobacilli microbiota. In addition, the mean lactobacilli/total bacterial mass (TBM) ratio in the vaginal samples was significantly lower in the unexplained infertility patients (38.2% vs.76.3%; p = 0.001). Conclusion The present study results supported the role of vaginal and endometrial microbiota derangements in unexplained infertility. Many time-consuming and invasive methods are currently used in the diagnosis of infertility. Our study showed that the quantitative determination of lactobacilli/TBM ratio in vaginal specimens, a less invasive and easily obtainable method, could be used as a diagnostic test during the workup of couples with unexplained infertility.tr
dc.identifier.doi10.1111/jog.15060en_US
dc.identifier.endpage139tr
dc.identifier.issue1tr
dc.identifier.pmid34657369en_US
dc.identifier.scopus2-s2.0-85116983294en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage129tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14034
dc.identifier.volume48tr
dc.identifier.wosWOS:000707718700001en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Editör Denetimli Dergide Makaletr
dc.rightsinfo:eu-repo/semantics/closedAccesstr
dc.titleAssessment of vaginal and endometrial microbiota by real?time PCR in women with unexplained infertilityen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
J of Obstet and Gynaecol - 2021 - Sezer - Assessment of vaginal and endometrial microbiota by real‐time PCR in women with.pdf
Boyut:
1.18 MB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Cerrahi Tıp Bilimleri Bölümü Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu