The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line

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dc.authoridOzdemir, Ercan/0000-0001-8231-1053
dc.authoridTaskiran, Ahmet Sevki/0000-0002-5810-8415
dc.contributor.authorTaskiran, Ahmet Sevki
dc.contributor.authorErgul, Mustafa
dc.contributor.authorGunes, Handan
dc.contributor.authorOzturk, Aysegul
dc.contributor.authorSahin, Bilal
dc.contributor.authorOzdemir, Ercan
dc.date.accessioned2024-10-26T18:04:15Z
dc.date.available2024-10-26T18:04:15Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractRecent studies have shown that proton pump inhibitors have positive effects on the nervous system. However, its effect on epileptic seizure and neuronal damage are still unclear. In this study, it was aimed to investigate the effect of pantoprazole on pentylenetetrazole-induced epileptic seizures in rats and neurotoxicity in the SH-SY5Y cell line. Animals were divided into three groups: control, saline (1 mL/kg serum physiologic), and pantoprazole (10 mg/kg). Pentylenetetrazole (45 mg/kg) was given to induce a seizure and a passive avoidance test trial was carried out to evaluate memory function. 8-hydroxy-2 '-deoxyguanosine (8-OHdG), caspase-3, and brain-derived neurotrophic factor (BDNF) levels were measured in the brain by commercial kits. SH-SY5Y cells were treated with saline or pantoprazole for one hour, and then pentylenetetrazole (30 mu m) was added to the medium to induce neurotoxicity. After 24 h, cell viability, total antioxidant, total oxidant status, and apoptosis were measured in SH-SY5Y cells. It was found that pantoprazole treatment postponed epileptic seizure onset, protected memory, reduced 8-OHdG, caspase-3, and also increased BDNF in the brain. In addition, it blocked pentylenetetrazole toxicity, apoptosis, increased antioxidant, and decreased oxidant status in SH-SY5Y cells. Pantoprazole significantly improved seizure, oxidative stress, and apoptosis. Thus, pantoprazole could be used as a supportive therapeutic agent in epilepsy.
dc.identifier.doi10.1007/s10571-020-00956-6
dc.identifier.endpage183
dc.identifier.issn0272-4340
dc.identifier.issn1573-6830
dc.identifier.issue1
dc.identifier.pmid32862257
dc.identifier.scopus2-s2.0-85089977359
dc.identifier.scopusqualityQ1
dc.identifier.startpage173
dc.identifier.urihttps://doi.org/10.1007/s10571-020-00956-6
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28835
dc.identifier.volume41
dc.identifier.wosWOS:000563717600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer/Plenum Publishers
dc.relation.ispartofCellular and Molecular Neurobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpilepsy
dc.subjectPentylenetetrazole
dc.subjectPantoprazole
dc.subjectRats
dc.subjectSH-SY5Y
dc.titleThe Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line
dc.typeArticle

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