Anticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell line

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dc.authorid0000-0002-2778-8059tr
dc.contributor.authorTaştemur, Şeyma
dc.contributor.authorHacısüleyman, Levent
dc.contributor.authorKarataş, Özhan
dc.contributor.authorYulak Fatih
dc.contributor.authorAtaseven Hilmi
dc.date.accessioned2024-03-07T05:18:34Z
dc.date.available2024-03-07T05:18:34Z
dc.date.issued28.04.2023tr
dc.departmentEğitim Bilimleri Enstitüsütr
dc.description.abstractColorectal cancer is the third most lethal and fourth most commonly diagnosed cancer worldwide. Sinapic acid, a derivative of hydroxycinnamic acid, is a promising phytochemical exhibiting numerous pharmacological activities in various systems. It is a substantial chain-breaking antioxidant that operates as a radical scavenger. The aim of this research was to investigate the antiproliferative effect of sinapic acid on the HT-29 cell line besides the mechanisms underlying this activity. The effect of sinapic acid on the viability of HT-29 cell line was investigated using XTT assay. The levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG were measured using ELISA. Gamma-H2AX and cytochrome c expressions were assessed semiquantitatively using immunofluorescence staining. Sinapic acid at 200 µm and higher doses produced a significant antiproliferative effect on HT-29 cells. The IC50 value was found to be 317.5 µm for 24 h. Sinapic acid (317.5 µm) significantly elevated cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG levels. The levels of gamma-H2AX foci are significantly higher, while the levels of cytochrome c are lower in sinapic acid-treated HT-29 cells. These results indicate that sinapic acid has antiproliferative, apoptotic, and genotoxic effects on colon cancer cells.tr
dc.identifier.doi10.1139/cjpp-2022-0523en_US
dc.identifier.pmid36996488en_US
dc.identifier.scopus2-s2.0-85164230751en_US
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14805
dc.identifier.wosWOS:000983071100001en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Editör Denetimli Dergide Makaletr
dc.rightsinfo:eu-repo/semantics/closedAccesstr
dc.subjectSinapic acid, Colorectal Cancer, HT-29 Cell Line, Antiproliferative Effecttr
dc.titleAnticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell lineen_US
dc.typeArticleen_US

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