Experimental and theoretical insights about the effect of some newly designed azomethine group?contained macroheterocycles on oxidative stress and DNA repair gene profiles in neuroblastoma cell lines

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dc.authorid0000-0002-0562-7457tr
dc.contributor.authorCinar, Gulcihan
dc.contributor.authorAgbektas, Tugba
dc.contributor.authorHuseynzada, Alakbar
dc.contributor.authorAliyeva, Gunel
dc.contributor.authorAghayev, Mirjavid
dc.contributor.authorHasanova, Ulviyya
dc.contributor.authorKaya, Savas
dc.contributor.authorChtita, Samir
dc.contributor.authorNour, Hassan
dc.contributor.authorTas, Ayca
dc.contributor.authorSilig, Yavuz
dc.date.accessioned2024-03-07T05:56:32Z
dc.date.available2024-03-07T05:56:32Z
dc.date.issued2023/8/5tr
dc.departmentTıp Fakültesitr
dc.description.abstractWe report herein the synthesis of new azomethine group containing forty-, sixty-eight- and seventy- two-membered macroheterocyclic compounds and the investigation of their activity against the neurob- lastoma cell line. The synthesis of targeted compounds was done by condensation of dialdehydes with polyamines and their structures were investigated by 1 H and 13 C NMR and MALDI MS methods. Anti- cancer activity of these newly synthesized molecules was studied in the neuroblastoma (SH-SY5Y) cell line at eight different concentrations (1–100 μg/ml) for 24 h, 48 hrs and 72 h by MTT method. In ad- dition to it, oxidative stress (GPX1, PRDX1, CAT, SOD1, NQO1) and DNA repair (ATR, ERCC1, CDKN1A, PRKDC) gene profiles were also investigated by RT-PCR method. It was found that all synthesized compounds showed the highest activity after 72 h of incubation. PRDX1 gene expression in the case of all investi- gated compounds was found to be higher than the control group, whereas ABCB1 (MDR) gene expression increased only in the case of molecule 1. Results also demonstrate that the expression levels of GPX and SOD1 genes were high in the case of molecule 2, whereas molecule 1 manifested low expression levels of ERCC1, ATR, CDKN1A, PRKDC, GPX1, ABCB1(MDR), CAT, SOD1 and NQO1 genes. Along with experimen- tal studies, theoretical studies were also carried out. The String v11 program was used to determine the interaction of proteins involved in oxidative stress and DNA repair mechanisms with other proteins. The results of the molecular docking analysis were found to be in good agreement with the experiments.tr
dc.identifier.doi10.1016/j.molstruc.2023.135432en_US
dc.identifier.issn0022-2860
dc.identifier.scopus2-s2.0-85151807183en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage135432tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14834
dc.identifier.volume1285tr
dc.identifier.wosWOS:000982506200001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElseviertr
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/closedAccesstr
dc.subjectNeuroblastoma cancertr
dc.subjectAzomethine group-contained compoundstr
dc.subjectDNA repairtr
dc.subjectOxidative stresstr
dc.subjectMolecular dockingtr
dc.subjectGene expressiontr
dc.titleExperimental and theoretical insights about the effect of some newly designed azomethine group?contained macroheterocycles on oxidative stress and DNA repair gene profiles in neuroblastoma cell linesen_US
dc.typeArticleen_US

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