The targeted photodynamic therapy of breast cancer with novel AS1411-indium(III) phthalocyanine conjugates

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dc.authoridYILDIZ, Esma/0000-0002-8094-8277
dc.authoridErdem Gursan, K. Arzum/0000-0002-4375-8386
dc.contributor.authorAkkus, Nurefsan
dc.contributor.authorEksin, Ece
dc.contributor.authorSahin, Gamze
dc.contributor.authorYildiz, Esma
dc.contributor.authorBagda, Efkan
dc.contributor.authorAltun, Ahmet
dc.contributor.authorBagda, Esra
dc.date.accessioned2024-10-26T18:09:16Z
dc.date.available2024-10-26T18:09:16Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn the study, the novel indium(III) phthalocyanines containing 4-mercaptopyridine groups as substituents on the peripheral (Pc3) or non-peripheral (Pc8) tetra-and peripheral octa (Pc13) positions were synthesized. After then, the synthesized phthalocyanines were converted to their water-soluble derivatives that are Pc5, Pc10, and Pc15 by the quaternization of the nitrogen atoms on the substituents. These new phthalocyanines have been characterized by various spectroscopic methods such as FT-IR, 1H-NMR, elemental analysis, UV-vis, MALDI-TOF mass spectra. Photochemical properties were also investigated for the synthesized phthalocyanines. In this study; quaternized derivatives were studied in DMSO and aqueous solutions, while non-quaternized derivatives were studied only in DMSO. The interaction between AS1411 and three phthalocyanines (Pc5, Pc10, and Pc15) was investigated using spectroscopic methods and electrochemical impedance spectroscopy technique combined with pencil graphite electrodes (PGEs). The cytotoxic activity of AS1411 and each phthalocyanine was investigated in the MCF-7 and doxorubicin (Dox) resistant MCF-7/DOX-R cell lines in the presence and absence of Dox. The photodynamic therapy (PDT) activity of Pc5, Pc10, and Pc15 and their AS1411 conjugates was investigated on MCF-7, MCF-7/DOX-R and MCF-10A cells. The IC50 values for MCF-7 cells were found as 1.1, 0.46, and 0.46 mM for Pc5 and 0.58, 0.34, and 0.32 mM for Pc5-AS1411 conjugate in the absence of irradiation and radiation with 7 J and 20 J. The values for MCF-7/DOX-R cells were found 0.81, 0.30, and 0.11 mM for Pc5 and 0.39, 0.12, and 0.15 mM for Pc5-AS1411 conjugate without irradiation and radiation with 7 J and 20 J, respectively. Apoptosis and cell imaging studies showed that conjugation of the phthalocyanines with AS1411 induced apoptosis in both cell lines and increases the intracellular entry of phthalocyanine Pc5.
dc.description.sponsorshipTUBITAK; Scientific Research Project Fund of Sivas Cumhuriyet University [CUEBAP-ECZ-074]; [118Z204]
dc.description.sponsorshipThis work was supported by the project TUBITAK 118Z204 and the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number CUEBAP-ECZ-074. N.A., G.S ,, E.E and E.Y acknowledge a project scholarship (TUBITAK Project no. 118Z204) .
dc.identifier.doi10.1016/j.molstruc.2024.137718
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopus2-s2.0-85185201377
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2024.137718
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30038
dc.identifier.volume1305
dc.identifier.wosWOS:001198277900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Molecular Structure
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPhthalocyanine
dc.subjectIndium
dc.subjectSinglet oxygen
dc.subjectPhotodynamic therapy
dc.subjectElectrochemical impedance spectroscopy
dc.titleThe targeted photodynamic therapy of breast cancer with novel AS1411-indium(III) phthalocyanine conjugates
dc.typeArticle

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