Biocompatibility evaluation of cigarette and carbon papers used in repair of traumatic tympanic membrane perforations: experimental study

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dc.contributor.authorAltuntas, Emine Elif
dc.contributor.authorSumer, Zeynep
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:03:12Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:03:12Z
dc.date.issued2013
dc.department[Altuntas, Emine Elif] Cumhuriyet Univ, Dept Otorhinolaryngol, Sch Med, TR-58140 Sivas, Turkey -- [Altuntas, Emine Elif] Univ Cumhuriyet, Dept Ear Nose & Throat Educ, Sivas, Turkey -- [Altuntas, Emine Elif] Univ Cumhuriyet, Fac Med, Res Hosp, Sivas, Turkey -- [Sumer, Zeynep] Univ Cumhuriyet, Dept Microbiol, Fac Med, Sivas, Turkeyen_US
dc.description.abstractThe purposes of this study were to investigate the biocompatibility of two different paper patches (carbon and cigarette papers) and compare the adhesion and proliferation features of L929 fibroblast cells by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT Test) test and scanning electron microscopy (SEM). In this study, time-dependent cytotoxic effects of cigarette and carbon papers used in repairing small traumatic TM perforations were investigated in vitro by using MTT test. And also adhesion and spreading of cells over disk surface were observed by SEM. Cytotoxicity test carried out by MTT analysis on leakage products collected from two types of paper patches at the end of 24 and 48 h revealed no cytotoxicity (P > 0.05). In SEM studies, it was observed that cells started to proliferate over disk surface as a result of 48-h incubation, and SEM revealed that the cell proliferation over cigarette paper was more compared to the one over carbon paper. We believe that this is the first study where biocompatibility and adhesion features of carbon and cigarette paper have been studied by using L929 fibroblast cell culture. As a result, biocompatibility of cigarette paper and also whether cigarette paper was superior to carbon paper in cell attachment and biocompatibility were studied. It was found, by MTT test and SEM test, that cigarette paper had a higher biocompatibility and cell attachment, and thus cigarette paper should be the patch to be preferred in cases where TM perforations are repaired by paper-patch method.en_US
dc.identifier.doi10.1007/s00405-012-1934-6en_US
dc.identifier.endpage86en_US
dc.identifier.issn0937-4477
dc.identifier.issue1en_US
dc.identifier.pmid22271281en_US
dc.identifier.scopus2-s2.0-84872349953en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage81en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00405-012-1934-6
dc.identifier.urihttps://hdl.handle.net/20.500.12418/8912
dc.identifier.volume270en_US
dc.identifier.wosWOS:000313019700012en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofEUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTympanic membraneen_US
dc.subjectTraumatic perforationen_US
dc.subjectPaper patchesen_US
dc.subjectBiocompatibilityen_US
dc.subjectMTT testen_US
dc.subjectScanning electron microscopyen_US
dc.titleBiocompatibility evaluation of cigarette and carbon papers used in repair of traumatic tympanic membrane perforations: experimental studyen_US
dc.typeArticleen_US

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