Synthesis of New 1-Aryl-2-(3,5-dimethylpyrazol-1-yl)ethanone Oxime Ether Derivatives and Investigation of Their Cytotoxic Effects
Ghoneim, Mohammed M.
Abdelgawad, Mohamed A.
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CitationMehmet Abdullah Alagöz1,Arzu Karakurt1,Ceylan Hepokur2,Emine Şalva3,Tijen Önkol4,Muhammed M. Ghoneim5,Mohamed A. Abdelgawad6ORSİD,Ahmet Hames7ORSİD,Hoon Kim8,*ORSİDvebijo Mathew9,* 1İnönü Üniversitesi Eczacılık Fakültesi Farmasötik Kimya Anabilim Dalı, Malatya 44280, Türkiye 2Eczacılık Fakültesi, Temel Eczacılık Bilimleri Anabilim Dalı, Sivas Cumhuriyet Üniversitesi, Sivas 58100, Türkiye 3İnönü Üniversitesi Eczacılık Fakültesi Farmasötik Biyoteknoloji Anabilim Dalı, Malatya 44280, Türkiye 4Eczacılık Bölümü, Eczacılık Fakültesi, Gazi Üniversitesi, Ankara 06100, Türkiye 5Eczacılık Uygulamaları Anabilim Dalı, Eczacılık Fakültesi, AlMaarefa Üniversitesi, Ad Diriyah 13713, Suudi Arabistan 6Farmasötik Kimya Bölümü, Eczacılık Fakültesi, Jouf Üniversitesi, Sakaka 72341, Suudi Arabistan 7Eczacılık ve Endüstriyel Eczacılık Bölümü, Eczacılık Fakültesi, Taif Üniversitesi, PO Box 11099, Taif 21944, Suudi Arabistan 8Eczacılık Bölümü ve Yaşam Farmasötik Bilimleri Araştırma Enstitüsü, Sunchon Ulusal Üniversitesi, Suncheon 57922, Kore 9Farmasötik Kimya Bölümü, Amrita Eczacılık Okulu, Amrita Vishwa Vidyapeetham, AIMS Sağlık Bilimleri Kampüsü, Kochi 682 041, Hindistan
: In this study, 12 new 1-aryl-2-(3,5-dimethylpyrazol-1-yl)ethanone oxime ether derivatives were designed and synthesized to investigate their cytotoxic effects. The in vitro cytotoxic activities of the compounds were evaluated against cervix, colon, breast, glioma, neuroblastoma, and lung cancer cell lines, as well as a healthy cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazo lium bromide (MTT) assays with 5-fluorouracil (5-FU) as the reference compound. Compound 5f (IC50 = 5.13 µM) was found to be more effective than 5-FU (IC50 = 8.34 µM) in the C6 cancer cell line, and it had no cytotoxic effect on the L929 healthy cell line. Flow cytometry was used to investigate the mechanism of action of compound 5f on the cell cycle of the C6 cell line. The analysis showed that cell death was significantly due to apoptosis. These results indicate that compound 5f induces cell cycle arrest, and may be effective in treating glioma.